Control of Respiration and Nitrogen Fixation by Oxygen and Adenine Nucleotides in N-grown Free

Abstract

SUMMARY: Oxygen uptake by (NCIB 8003) grown in continuous culture without fixed nitrogen and with a low mannitol concentration (2°5 g./1.) and treated with lysozyme and EDTA was inhibited by ATP but not by ADP; ADP frequently prevented inhibition by ATP. In preparations obtained by disrupting bacteria suspended in a mixture of defatted bovine serum albumin, sucrose and MgCl in the French press, ATP inhibited oxygen uptake with either sodium succinate or sodium isocitrate as substrates and ADP prevented this inhibition; oxygen uptake with glucose-6-phosphate was inhibited by ATP or ADP. A form of respiratory control by nucleotides may thus occur in Azotobacter. Acetylene reduction (a measure of nitrogenase activity) by bacteria treated with lysozyme and EDTA was inhibited by ATP; this was attributed to inhibition of oxygen uptake by ATP causing inhibition of nitrogenase by oxygen. High oxygen solution rates inhibited nitrogenase in whole bacteria or in bacteria treated with lysozyme and EDTA; when the oxygen solution rate was lowered nitrogenase functioned immediately. These observations are probably expressions of processes which protect nitrogenase in whole bacteria from damage by oxygen.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-60-3-393
1970-03-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/60/3/mic-60-3-393.html?itemId=/content/journal/micro/10.1099/00221287-60-3-393&mimeType=html&fmt=ahah

References

  1. Aldridge W. N., Street B. W. 1968; Mitochondria from brown adipose tissue.. Biochemical Journal 107:315
    [Google Scholar]
  2. Baker K. 1968; Low cost continuous culture apparatus.. Laboratory Practice 17:817
    [Google Scholar]
  3. Chance B., Hollunger G. 1961; The interaction of energy and electron transfer reactions in mitochondria.. Journal of Biological Chemistry 236:1945
    [Google Scholar]
  4. Cooper C. M., Fernstrom G. A., Miller S. A. 1944; Performance of agitated gas-liquid contractors.. Industrial and Engineering Chemistry 36:504
    [Google Scholar]
  5. Dalton H., Postgate J. R. 1967; Inhibition of growth of Azotobacter by oxygen.. Journal of General Microbiology 48:v
    [Google Scholar]
  6. Dalton H., Postgate J. R. 1969; Effect of oxygen on growth of Azotobacter chroococcum in batch and continuous cultures.. Journal of General Microbiology 54:463
    [Google Scholar]
  7. Dilworth M. J. 1966; Acetylene reduction by N2-fixing preparations of Clostridium pasteurianum. . Biochimica et Biophysica Acta 127:285
    [Google Scholar]
  8. Fahmy A. R., Walsh E. O’F. 1952; The quantitative determination of dehydrogenase activity in cell suspensions.. Biochemical Journal 51:55
    [Google Scholar]
  9. Ishikawa S., Lehninger A. L. 1962; Reconstitution of oxidative phosphorylation in preparations of Micrococcus lysodeikticus. . Journal of Biological Chemistry 237:2401
    [Google Scholar]
  10. Johnson E. J., Johnson M. K. 1961; Alternate pathways of glucose metabolism in Azotobacter agilis. . Proceedings of the Society for Experimental Biology and Medicine 108:728
    [Google Scholar]
  11. Kelly M. 1969; Some properties of purified nitrogenase from Azotobacter chroococcum. . Biochimica et Biophysica Acta 171:9
    [Google Scholar]
  12. Klingenberg M., Schollmeyer P. 1960; Zur Reversibilität der oxidativen Phosphorylierung.. Biochemische Zeitschrift 333:335
    [Google Scholar]
  13. Lehninger A. L. 1964 The Mitochondria139 New York: W. A. Benjamin, Inc.;
    [Google Scholar]
  14. Mahler H. R., Cordes E. H. 1966 Biological Chemistry531 New York: Harper & Row.;
    [Google Scholar]
  15. Mortenson L. E., Wilson P. W. 1954; Initial stages in the breakdown of carbohydrates by the Azotobacter vinelandii. . Archives of Biochemistry and Biophysics 53:425
    [Google Scholar]
  16. Nielsen S. O., Lehninger A. L. 1955; Phosphorylation coupled to the oxidation of ferrocytochrome c.. Journal of Biological Chemistry 215:555
    [Google Scholar]
  17. Postgate J. R. 1969; Royal Society Discussion Meeting on nitrogen fixation: discussion.. Proceedings of the Royal Society B 172:355
    [Google Scholar]
  18. Revsin B., Brodie A. F. 1967; An effect of inorganic phosphate and AMP at the third phosphorylative site of the respiratory chain of Mycobacterium phlei. . Biochemical and Biophysical Research Communications 28:635
    [Google Scholar]
  19. Schindler J., Schlegel H. G. 1969; Regulation of glucose-6-phosphate dehydrogenase activity in several bacteria by ATP.. Archiv für Mikrobiologie 66:69
    [Google Scholar]
  20. Schöllhorn R., Burris R. H. 1967; Acetylene as a competitive inhibitor of N2 fixation.. Proceedings of the National Academy of Sciences of the United States of America 58:213
    [Google Scholar]
  21. Scocca J. J., Pinchot G. B. 1965; Stimulation of DPNH oxidation by inorganic phosphate in phosphorylating particles from Alkaligenes faecalis. . Federation Proceedings. Federation of American Societies for Experimental Biology 24:554
    [Google Scholar]
  22. Still G. G., Wang C. H. 1964; Glucose catabolism in Azotobacter vinelandii. . Archives of Biochemistry and Biophysics 105:126
    [Google Scholar]
  23. Williams A. M., Wilson P. W. 1964; Adaptation of Azotobacter cells to tricarboxylic acid substrates.. Journal of Bacteriology 67:353
    [Google Scholar]
  24. Worcel A., Goldman D. S., Cleland W. W. 1965; An allosteric reduced nicotinamide adenine dinucleotide oxidase from Mycobacterium tuberculosis. . Journal of Biochemical Chemistry 240:3399
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-60-3-393
Loading
/content/journal/micro/10.1099/00221287-60-3-393
Loading

Data & Media loading...

Most cited Most Cited RSS feed