@article{mbs:/content/journal/micro/10.1099/00221287-60-3-387, author = "Rastogi, A. K. and Sagar, P. and Agarwala, S. C.", title = "Production of Single-walled Cysts of Schizopyrenus russelli by Sonication and their Behaviour Towards Excystment-inducing and Excystment-blocking Agents", journal= "Microbiology", year = "1970", volume = "60", number = "3", pages = "387-392", doi = "https://doi.org/10.1099/00221287-60-3-387", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-60-3-387", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "SUMMARY: Ultrasonic treatment of double-walled cysts of Schizopyrenus russelli led to the removal of the outer wall. These single-walled cysts excysted readily in aqueous extract of Escherichia coli, as did normal double-walled cysts. There was a marked delay in the excystment of single-walled cysts obtained by ultrasonic treatment of the normal cysts treated with p-chloromercuric benzoate (p-CMB) or emetine, similar to that obtained with normal cysts. The action of p-CMB was annulled by reduced glutathione. These findings suggest that the sites of action of excystment-inducing and excystment-blocking agents are on or inside the inner cyst wall and that these agents are able to permeate the outer cyst wall.", }