SUMMARY: Hydrostatic pressure initiated germination of bacterial spores in nutrient-free media. Those spores which were most dormant towards chemical germinants at I atmosphere pressure were also the most resistant to germination by pressure treatment. Germination by high pressure treatment was characterized by temperature and pH optima, like germination at atmospheric pressure. Germination initiated by pressure was inhibited by metabolic poisons and was potentiated by low concentrations of various nutrients including some of those which are normally germinative (at higher concentrations) at atmospheric pressure. In particular, L-alanine and closely related α-amino acids, but not their breakdown products, potentiated germination initiated by pressure. Study of potentiation by D-alanine (which strongly inhibits germination initiated by L-alanine at I atmosphere pressure) revealed that high pressures caused an increase in the rate of racemization of alanine by spores. Germination by pressure probably resulted from acceleration of some germination reaction which is normally negligibly slow at a pressure of I atmosphere, and also from an increase in permeability of some barrier within the spore to L-alanine and related α-amino acids.
CloustonJ. G.,
WillsP. A.1969; Initiation of germination and inactivation of Bacilluspumilis spores by hydrostatic pressure.. Journal of Bacteriology 97:684
DouglasH. E.,
JohnsonF. H.1951; The influence of hydrostatic pressure and pH on the rate of lysis of Micrococcus lysodeikticus by lysozyme.. Archives of Biochemistry and Biophysics 31:41
GonikbergM. G.,
ProkhorovaN. I.,
yanovB. S.1968; Effect of high pressure on the stereospecificity of enzyme catalysis.. Izvestiya Akademii Nauk SSSR, Ser. khim 12:2841
HillsG. W.1949; Chemical factors in the germination of spore-bearing aerobes. The effects of amino-acids on the germination of Bacillus anthracis, with some observations on the relation of optical form to biological activity.. Biochemical Journal 45:363
LandauJ. V.,
PeabodyR. A.1963; Endogenous adenosine triphosphate levels in human amnion cells during application of high hydrostatic pressure.. Experimental Cell Research 29:54
RodeL. J.,
FosterJ. W.1960; Mechanical germination of bacterial spores.. Proceedings of the National Academy of Sciences of the United States of America 46:118
WerbinH.,
McLaelenA. D.1951a; The effect of high pressure on the rates of proteolytic hydrolysis I. Chymotrypsin.. Archives of Biochemistry and Biophysics 31:285
WerbinH.,
McLarenA. D.1951b; The effect of high pressure on the rates of proteolytic hydrolysis II. Trypsin.. Archives of Biochemistry and Biophysics 31:325
WoeseC. R.,
VaryJ. C.,
HalvorsonH. O.1968; A kinetic model for bacterial spore germination.. Proceedings of the National Academy of Sciences of the United States of America 59:869