1887

Abstract

SUMMARY: The dry weight of starved of an energy source decreased exponentially. The protein, RNA, DNA and endogenous respiration decreased rapidly during the first few hours of starvation and reached about 25% of their original values after 5 days starvation. The carbohydrate content of the mould only decreased after the first 2 days of starvation. About 60% of the carbon lost during autolysis was oxidized to carbon dioxide. Lysis of organelles in individual hyphal compartments occurred synchronously and did not seem to involve autophagy. Autolysis of separate compartments in the same hyphae was not synchronized. Ribosomes were rapidly degraded but membranes were particularly resistant to breakdown. Intra-hyphal hyphae were observed. In the final stages of autolysis the culture consisted largely of empty hyphal walls. A proportion of cytologically normal hyphal compartments were present at all stages, suggesting that maintenance and/or cryptic growth of some hyphae (or compartments) occurred at the expense of others.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-60-2-239
1970-02-01
2022-01-17
Loading full text...

Full text loading...

/deliver/fulltext/micro/60/2/mic-60-2-239.html?itemId=/content/journal/micro/10.1099/00221287-60-2-239&mimeType=html&fmt=ahah

References

  1. Beaven G. H., Holiday E. R., Dawson G. A. 1965 The Nucleic Acids Ed. by Chargaff E., Davidson J. N. I493 London: Academic Press.;
    [Google Scholar]
  2. Ben-Hamida F., Schlessinger D. 1966; Synthesis and breakdown of ribonucleic acids in Escherichia coli starving for nitrogen.. Biochimica et Biophysica Acta 119:183
    [Google Scholar]
  3. Berliner M. D., Duff R. H. 1965; Ultrastructure of Armillaria mellea hyphae.. Canadian Journal of Botany 43:171
    [Google Scholar]
  4. Blum J. J., Buetow D. E. 1963; Biochemical changes during acetate deprivation of Euglena gracilis.. Experimental Cell Research 29:407
    [Google Scholar]
  5. Bracker C. E. 1966; Ultrastructural aspects of sporangiospore formation in Gilbertella persicaria.. The Fungus Spore39 London: Butterworths.;
    [Google Scholar]
  6. Bracker C. E. 1967; Ultrastructure of fungi.. Annual Review of Phytopathology 5:343
    [Google Scholar]
  7. Brandes D., Buetow D. E., Bertini F., Malkoff D. B. 1964; Roles of lysosomes in cellular lytic processes. I. Effect of carbon starvation in Euglena gracilis.. Experimental Molecular Pathology 3:583
    [Google Scholar]
  8. Burleigh I. G., Dawes E. A. 1967; Studies on the endogenous metabolism and senescence of starved Sarcina lutea.. Biochemical Journal 102:236
    [Google Scholar]
  9. Calonge F. D. 1968; Origin and development of intrahyphal hyphae in Sclerotinia fructigena.. Mycologia 60:932
    [Google Scholar]
  10. Calonge F. D., Fielding A. H., Byrde R. J. W. 1969; Multivesicular bodies in Sclerotinia fructigena and their possible relation to extracellular enzyme secretion.. Journal of General Microbiology 55:177
    [Google Scholar]
  11. Chan C., Stephen R. C. 1967; Intrahyphal hyphae in the genus Linderina.. Canadian Journal of Botany 45:1995
    [Google Scholar]
  12. Dawes E. A., Ribbons D. W. 1964; Some aspects of the endogenous metabolism of bacteria.. Bacteriological Reviews 28:126
    [Google Scholar]
  13. DeDuve C., Wattiaux R. 1966; Functions of lysosomes.. Annual Review of Physiology 28:435
    [Google Scholar]
  14. Duguid J. P., Wilkinson J. F. 1961; Environmentally induced changes in bacterial morphology.. Symposia of the Society for General Microbiology 11:69
    [Google Scholar]
  15. Elliott A. M. 1965; Primary lysosomes in Tetrahymena pyriformis.. Science, New York 149:640
    [Google Scholar]
  16. Georgiev G. P., Chentzov J. S. 1963; An electron microscope study of the ultrastructure of isolated nuclei subjected to salt extraction.. Biophysika 8:50
    [Google Scholar]
  17. Hyde J. M., Wilkinshaw C. H. 1966; Ultrastructure of basidiospores and mycelium of Lenzites saepiaria.. Journal of Bacteriology 92:1218
    [Google Scholar]
  18. Jensen W. A. 1962 Botanical Histochemistry San Francisco: Freeman & Co.;
    [Google Scholar]
  19. Lowry R. J., Sussman A. S. 1966; Intra-hyphal hyphae in ‘clock’ mutants of Neurospora.. Mycologia 58:541
    [Google Scholar]
  20. McLean J., Gahan P. B. 1968; Hydrolase activities in differentiating plant tissue.. Biochemical Journal 111:10
    [Google Scholar]
  21. Mandelstam J. 1960; The intracellular turnover of protein and nucleic acid and its role in biochemical differentiation.. Bacteriological Reviews 24:289
    [Google Scholar]
  22. Marchant R., Peat A., Banbury G. H. 1967; The ultrastructural basis of hyphal growth.. New Phytologist 66:623
    [Google Scholar]
  23. Matile P. 1966; Inositol deficiency resulting in death: an explanation of its occurrence in Neurospora crassa.. Science, New York 151:1
    [Google Scholar]
  24. Morton A. G., Dickerson A. G. F., England D. J. F. 1960; Changes in enzyme activity of fungi during nitrogen starvation.. Journal of Experimental Botany 2:116
    [Google Scholar]
  25. Nagasaki S. 1968; Physiological aspects of various enzymes in relation to the culture age of Aspergillus niger mycelia.. Journal of General and Applied Microbiology, Tokyo 14:147
    [Google Scholar]
  26. Osumi M., Katoh T. 1967; Oxygen-induced formation of mitochondrial membrane matrix in respiration deficient yeast.. Japan Women’s University Journal 14:67
    [Google Scholar]
  27. Postgate J. R. 1967; Viability measurement and survival of microbes under minimum stress.. Advances in Microbiological Physiology 1:1
    [Google Scholar]
  28. Postgate J. R., Hunter J. R. 1962; The survival of starved bacteria.. Journal of General Microbiology 29:233
    [Google Scholar]
  29. Revel J. P., Ito S., Fawcett D. W. 1968; Electron micrographs of myelin figures of phospholipids simulating intracellular membranes.. Journal of Biophysical and Biochemical Cytology 4:495
    [Google Scholar]
  30. Righelato R. C., Trinci A. P. J., Pirt S. J., Peat A. 1968; The influence of maintenance energy and growth rate on the metabolic activity, morphology and conidiation of Penicillium chrysogenum.. Journal of General Microbiology 50:399
    [Google Scholar]
  31. Ruinen J., Deinema M. H., DerScheer C. 1968; Cellular and extracellular structures in Cryptococcus laurentii.. Canadian Journal of Microbiology 14:1133
    [Google Scholar]
  32. Ryan F. J. 1955; Spontaneous mutation in non-dividing bacteria.. Genetics, Princeton 40:726
    [Google Scholar]
  33. Schlessinger D., Hamida F. 1966; Turnover of protein in Escherichia coli starving for nitrogen.. Biochimica et Biophysica Acta 119:171
    [Google Scholar]
  34. Smith D. G., Marchant R. 1968; Lipid inclusions in the vacuole of Saccharomyces cerevisiae.. Archiv fur Mikrobiologie 60:340
    [Google Scholar]
  35. Strange R. E., Dark F. A., Ness A. G. 1961; The survival of stationary phase cells of Aerobacter aerogenes stored in aqueous suspension.. Journal of General Microbiology 25:61
    [Google Scholar]
  36. Tempest D. W., Strange R. E. 1966; Variation in content and distribution of magnesium, and its influence on survival in Aerobacter aerogenes grown in a chemostat.. Journal of General Microbiology 44:273
    [Google Scholar]
  37. Thomas T. D., Batt R. D. 1969; Degradation of cell constituents by starved Streptococcus lactis in relation to survival.. Journal of General Microbiology 58:347
    [Google Scholar]
  38. Thomas P. L., Isaac P. K. 1967; An electron microscope study of intravacuolar bodies in the uredia of wheat stem rust and in hyphae of other fungi.. Canadian Journal of Botany 45:1473
    [Google Scholar]
  39. Thornton R. M. 1968; The fine structure of Phycomyces. I. Autophagic vesicles.. Journal of Ultrastructure Research 21:269
    [Google Scholar]
  40. Trinci A. P. J. 1969; A kinetic study of the growth of Aspergillus nidulans and other fungi.. Journal of General Microbiology 57:11
    [Google Scholar]
  41. Venable J. H., Coggeshall R. 1965; A simplified lead citrate stain for use in electron microscopy.. Journal of Cell Biology 25:407
    [Google Scholar]
  42. Wilkinson J. F. 1958; The extracellular polysaccharides of bacteria.. Bacteriological Reviews 22:46
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-60-2-239
Loading
/content/journal/micro/10.1099/00221287-60-2-239
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error