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Volume 60, Issue 1

Effect of Starvation on the Viability and Cellular Constituents of Free

Abstract

SUMMARY: and grown on glucose + peptone + yeast-extract medium, degraded no endocellular carbohydrate, DNA and protein even in prolonged starvation. No significant qualitative changes in protein content during starvation were detected by disc gel electrophoresis of crude extracts. Both organisms had a high content of RNA (22% w/w) which was degraded on starvation. In RNA decreased linearly to 5% of the dry weight in 125 hr. With half the RNA was degraded in the first 24 hr of starvation after which time the decline was much slower. MgCl (33 mM) prevented RNA breakdown. During growth, the intracellular ATP concentration increased from 0.5 to 1.0 g./mg. dry wt, but began to decrease exponentially in the last generation before growth ceased because of glucose exhaustion. Intracellular ATP content correlated with viability determined by slide culture. The addition of 33 mM-MgCl to the starvation medium did not affect ATP content, but increased viability. On prolonged starvation (up to 7 days), populations whose viability had fallen to 3% possessed unimpaired ability to produce ATP from glucose; only after even longer starvation periods was this ability impaired.

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© Society for General Microbiology 1970
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1970-01-01
2024-04-19
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References

  1. BelaÏch J.-P., Senez J. C. 1965; Influence of aeration and of pantothenate on growth yields of Zymomonas mobilis. . Journal of Bacteriology 89:1195
     [Google Scholar]
  2. Binnie B., Dawes E. A., Holms W. H. 1960; Metabolism of Sarcina lutea. IV. Patterns of oxidative assimilation.. Biochimica et Biophysica Acta 40:237
     [Google Scholar]
  3. Bowen T. J., Dagley S., Sykes J. 1959; A ribonucleoprotein component of Escherichia coli. . Biochemical Journal 72:419
     [Google Scholar]
  4. Burleigh I. G., Dawes E. A. 1967; Studies on the endogenous metabolism and senescence of starved Sarcina lutea. . Biochemical Journal 102:236
     [Google Scholar]
  5. Ceriotti G. 1952; A microchemical determination of desoxyribonucleic acid.. Journal of Biological Chemistry 198:297
     [Google Scholar]
  6. Chaney A. L., Marbach E. P. 1962; Modified reagents for determination of urea and ammonia.. Clinical Chemistry 8:130
     [Google Scholar]
  7. Cole H. A., Wimpenny J. W. T., Hughes D. E. 1967; The ATP pool in Escherichia coli. I. Measurement of the pool using a modified luciferase assay.. Biochimica et Biophysica Acta 143:445
     [Google Scholar]
  8. Damoglou A. P., Dawes E. A. 1968; Studies on the lipid content and phosphate requirement of glucose- and acetate-grown Escherichia coli. . Biochemical Journal 110:775
     [Google Scholar]
  9. Davis B. J. 1964; Disk electrophoresis. II. Method and application to human serum proteins.. Annals of the New York Academy of Sciences 121:404
     [Google Scholar]
  10. Dawes E. A., Holms W. H. 1958; Apparatus for aeration and sampling of bacteria during growth and metabolic studies.. Nature, London 178:318
     [Google Scholar]
  11. Dawes E. A., Ribbons D. W. 1962; The endogenous metabolism of micro-organisms.. Annual Review of Microbiology 16:241
     [Google Scholar]
  12. Dawes E. A., Ribbons D. W. 1964; Some aspects of the endogenous metabolism of bacteria.. Bacteriological Reviews 28:126
     [Google Scholar]
  13. Dawes E. A., Ribbons D. W. 1965; Studies on the endogenous metabolism of Escherichia coli. . Biochemical Journal 95:332
     [Google Scholar]
  14. Dawes E. A., Ribbons D. W., Large P. J. 1966; The route of ethanol formation in Zymomonas mobilis. . Biochemical Journal 98:795
     [Google Scholar]
  15. Elsden S. R., Peel J. L. 1958; Metabolism of carbohydrates and related compounds.. Annual Review of Microbiology 12:145
     [Google Scholar]
  16. Entner N., Doudoroff M. 1952; Glucose and gluconic acid oxidation of Pseudomonas saccharophila. . Journal of Biological Chemistry 196:853
     [Google Scholar]
  17. Fleck A., Munro H. N. 1962; The precision of ultraviolet absorption measurements in the Schmidt-Thannhauser procedure for nucleic acid estimation.. Biochimica et Biophysica Acta 55:571
     [Google Scholar]
  18. Forrest W. W., Walker D. J. 1965; Synthesis of reserve materials for endogenous metabolism in Streptococcus faecalis. . Journal of Bacteriology 89:1448
     [Google Scholar]
  19. Friedemann T. E., Haugen G. E. 1943; Pyruvic acid. II. The determination of keto acids in blood and urine.. Journal of Biological Chemistry 147:415
     [Google Scholar]
  20. Gronlund A. F., Campbell J. J. R. 1965; Enzymatic degradation of ribosomes during endogenous respiration of Pseudomonas aeruginosa. . Journal of Bacteriology 90:1
     [Google Scholar]
  21. Harold F. M. 1960; Accumulation of inorganic polyphosphate in mutants of Neurospora crassa. . Biochimica et Biophysica Acta 45:172
     [Google Scholar]
  22. Huggett A., Nixon D. A. 1957; Use of glucose oxidase, peroxidase and o-dianisidine in determination of blood and urinary glucose.. Lancet ii:368
     [Google Scholar]
  23. Kaplan N. O., Qotti M. M. 1957; Enzymatic determination of ethanol.. Methods in Enzy-mology 3:253
     [Google Scholar]
  24. Large P. J., Dawes E. A. 1966; Endogenous metabolism of Zymomonas anaerobia and Peptococcus prevotii. . Ninth International Congress for Microbiology Moscow, 1966. Abstracts of papers 120
     [Google Scholar]
  25. Law J. H., Slepecky R. A. 1961; Assay of poly-β-hydroxybutyric acid.. Journal of Bacteriology 82:33
     [Google Scholar]
  26. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent.. Journal of Biological Chemistry 193:265
     [Google Scholar]
  27. McGill D. J. 1966 The carbohydrate metabolism of Zymomonas anaerobia. Ph.D. Thesis: University of Hull.;
     [Google Scholar]
  28. McGill D. J., Dawes E. A., Ribbons D. W. 1965; Carbohydrate metabolism and growth-yield coefficients of Zymomonas anaerobia. . Biochemical Journal 97:44
     [Google Scholar]
  29. Nelson N. 1944; A photometric adaptation of the Somogyi method for the determination of glucose.. Journal of Biological Chemistry 153:375
     [Google Scholar]
  30. Postgate J. R., Crumpton J. E., Hunter J. R. 1961; The measurement of bacterial viabilities by slide culture.. Journal of General Microbiology 24:15
     [Google Scholar]
  31. Shimwell J. L. 1937; Study of a new type of beer disease bacterium (Achromobacterium anaerobium spec, nov.) producing alcoholic fermentation of glucose.. Journal of the Institute of Brewing 43:507
     [Google Scholar]
  32. Shimwell J. L. 1950; Saccharomonas, a proposed new genus for bacteria producing a quantitative alcoholic fermentation of glucose.. Journal of the Institute of Brewing 56:179
     [Google Scholar]
  33. Stern I. G., Wang C. H., Gilmour C. M. 1960; Comparative catabolism of carbohydrates in Pseudomonas species.. Journal of Bacteriology 79:601
     [Google Scholar]
  34. Strange R. E., Dark F. A., Ness A. G. 1961; The survival of stationary phase Aerobacter aerogenes stored in aqueous suspension.. Journal of General Microbiology 25:61
     [Google Scholar]
  35. Strange R. E., Hunter J. R. 1967; Effect of magnesium on the survival of bacteria in aqueous suspension.. Microbial Physiology and Continuous Culture Ed. by Powell E. O., Evans C. G. T., Strange R. E., Tempest D. W. 102 London: Her Majesty’s Stationery Office.;
     [Google Scholar]
  36. Strange R. E., Shon M. 1964; Effect of thermal stress on viability and ribonucleic acid of Aerobacter aerogenes in aqueous suspension.. Journal of General Microbiology 34:99
     [Google Scholar]
  37. Strange R. E., Wade H. E., Dark F. A. 1963; Effect of starvation on adenosine triphosphate concentration in Aerobacter aerogenes. . Nature, London 199:55
     [Google Scholar]
  38. Thomas T. D. 1968 Metabolism of lactic acid bacteria Ph.D. Thesis: Massey University, New Zealand.;
     [Google Scholar]
  39. Thomas T. D., Batt R. D. 1968; Survival of Streptococcus lactis in starvation conditions.. Journal of General Microbiology 50:367
     [Google Scholar]
  40. Trevelyan W. D., Harrison J. S. 1952; Studies on yeast metabolism. I. Fractionation and microdetermination of all carbohydrates.. Biochemical Journal 50:298
     [Google Scholar]
  41. Wade H. E. 1961; The autodegradation of ribonucleoprotein in Escherichia coli. . Biochemical Journal 78:457
     [Google Scholar]
  42. Williamson D. H., Wilkinson J. F. 1958; The isolation and estimation of poly-β-hydroxy-butyrate inclusions of Bacillus species.. Journal of General Microbiology 19:198
     [Google Scholar]
  43. Yemm E. W., Cocking E. C. 1955; The determination of amino acids with ninhydrin.. Analyst, London 80:209
     [Google Scholar]
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Effect of Starvation on the Viability and Cellular Constituents of Zymomonas anaerobia and Zymomonas mobilis
Microbiology 60, 31 (1970); https://doi.org/10.1099/00221287-60-1-31
/content/journal/micro/10.1099/00221287-60-1-31
/content/journal/micro/10.1099/00221287-60-1-31
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