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Abstract
SUMMARY: The simultaneous uptake of CO2, H2, and O2 by non-proliferating cells of Hydrogenomonas facilis was studied by means of a new method employing ordinary Warburg vessels each provided with one stopper side-arm and one vent side-arm. H. facilis effected the same overall reaction as that carried out by certain anaerobically adapted green algae: 6H2 + 2O2 + CO2 → (CH2O) + 5H2O. In an atmosphere of CO2 and H2, no CO2 fixation accompanied the quantitative reduction of nitrate to nitrite by molecular H2; nor was there any change in concentration of bicarbonate or in total gas pressure when acetone, pyruvate, or α-ketoglutarate were added.
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© Society for General Microbiology 1952