The simultaneous uptake of CO, H, and O by non-proliferating cells of was studied by means of a new method employing ordinary Warburg vessels each provided with one stopper side-arm and one vent side-arm. effected the same overall reaction as that carried out by certain anaerobically adapted green algae: 6H + 2O + CO → (CHO) + 5HO. In an atmosphere of CO and H, no CO fixation accompanied the quantitative reduction of nitrate to nitrite by molecular H; nor was there any change in concentration of bicarbonate or in total gas pressure when acetone, pyruvate, or α-ketoglutarate were added.


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