and produced antibiotic substances when grown on organic materials which are used or could be used as manures. The addition of glucose increased the antibiotic titre in every case.

Antibiotics were also formed in autoclaved soils enriched with easily available carbon sources and inoculated with one of the following fungi: and two unidentified species of formed an antibiotic also on partially sterilized supplemented soil. The degree of antibiotic activity was decreased when soil cultures of were contaminated with other organisms from soil or from the air, but even gross contamination with natural soil at 25° did not destroy activity entirely until 11 days had elapsed. Loss of activity was smaller at lower temperatures.


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