A pseudomonad obtained from a nicotinic acid enrichment culture had a growth-factor requirement satisfied by high concentrations of nicotinic acid or by minute amounts of quinolinic acid, 3-hydroxyanthranilic acid, or nicotinamide. Nicotinic acid, kynurenin sulphate, and probably 3-hydroxyanthranilic acid, can serve as sole carbon sources. The oxidation of nicotinic acid is adaptive. The finding that low concentrations of nicotinic acid fail to satisfy the growth-factor requirement is discussed.

The paucity of records of auxotrophy in pseudomonads may reflect the selection exercised by isolation media devoid of growth factors. Pseudomonads requiring aneurin were isolated from enrichments with uracil and thymine. The utilization as carbon sources of metabolites of the anthranilic acid-tryptophan-nicotinic acid series may prove of taxonomic value in .


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