Summary: The diffusion rate of nisin through agar at pH 7·0 is increased by the addition of a non-ionic surface active agent to the medium. A linear relationship exists between log units nisin/ml. and inhibition zone depth over the range 100–5000 units/ml. By using seeded agar in small glass tubes and delaying growth of the test organism for 24 hr., a convenient form of assay is available for dealing with large numbers of samples.


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