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Volume 6, Issue 1-2

An Agar Diffusion Method for the Assay of Nisin Free

Abstract

Summary: The diffusion rate of nisin through agar at pH 7·0 is increased by the addition of a non-ionic surface active agent to the medium. A linear relationship exists between log units nisin/ml. and inhibition zone depth over the range 100–5000 units/ml. By using seeded agar in small glass tubes and delaying growth of the test organism for 24 hr., a convenient form of assay is available for dealing with large numbers of samples.

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  • Published Online:
© Society for General Microbiology, 1952
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/content/journal/micro/10.1099/00221287-6-1-2-60
1952-02-01
2024-04-25
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References

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An Agar Diffusion Method for the Assay of Nisin
Microbiology 6, 60 (1952); https://doi.org/10.1099/00221287-6-1-2-60
/content/journal/micro/10.1099/00221287-6-1-2-60
/content/journal/micro/10.1099/00221287-6-1-2-60
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