@article{mbs:/content/journal/micro/10.1099/00221287-6-1-2-47, author = "Crawford, Kathleen and Heatley, N. G. and Boyd, Pamela F. and Hale, C. W. and Kelly, B. K. and Miller, G. A. and Smith, Nancy", title = "Antibiotic Production by a Species of Cephalosporium", journal= "Microbiology", year = "1952", volume = "6", number = "1-2", pages = "47-59", doi = "https://doi.org/10.1099/00221287-6-1-2-47", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-6-1-2-47", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "Summary: The antibacterial activity of a strain of a Cephalosporium sp. was found to be due to a number of antibiotics. One group of these, named Cephalosporins P, is soluble in organic solvents and active only against Gram-positive bacteria. The P antibiotic(s) is produced in good yield in deep culture in a medium comprising corn steep liquor and glucose at an initial pH of 6·3. A high aeration rate assisted by adequate stirring is essential. Submerged cultures 72 hr old form convenient inocula. Under optimum conditions with 2% (v/v) of such an inoculum the maximum titre is reached after 47–50 hr. at 24°, and thereafter falls.", }