A Rapid Method for the Turbidimetric Assay of Antibiotics Free

Abstract

Summary: By using a culture of in its logarithmic phase of growth as test organism, it is possible to assay nisin satisfactorily with an incubation period of approximately half an hour. Penicillin, streptomycin, aureomycin and gramicidin cause a qualitatively similar response in the test organism. A similar technique could therefore be used to assay these antibiotics.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-6-1-2-14
1952-02-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/6/1-2/mic-6-1-2-14.html?itemId=/content/journal/micro/10.1099/00221287-6-1-2-14&mimeType=html&fmt=ahah

References

  1. Berridge N.J. 1949; Preparation of the antibiotic nisin. Biochem. J. 45:486
    [Google Scholar]
  2. Bond C.R., Davies O.L. 1948; The microbiological assay of penicillin by the turbidimetric method using Staphylococcus aureus. . Analyst 73:251
    [Google Scholar]
  3. Florey H.W., Chain E., Heatley N.G., Jennings M.A., Sanders A.G., Abraham E.P., Florey M.E. 1949 Antibiotics p. 147 London: Oxford University Press;
    [Google Scholar]
  4. Friedmann R., Beach S.A. 1951; New methods of assay for the antibiotic nisin. J. gen. Microbiol 5:v
    [Google Scholar]
  5. Hirsch A. 1950; The assay of the antibiotic nisin. J. gen Microbiol. 4:70
    [Google Scholar]
  6. Lee S.W., Foley E.J., Epstein J.A., Wallace J.H. 1944; Improvements in the turbidimetric assay for penicillin. J. biol. Chem. 152:485
    [Google Scholar]
  7. Matttck A.T.R., Hirsch A. 1947; Further observations on an inhibitory substance (nisin) from lactic streptococci. Lancet ii:5
    [Google Scholar]
  8. Patterson D.D. 1939 Statistical Technique in Agricultural Research Chap. 2. London: McGraw-Hill Book Co.Inc.;
    [Google Scholar]
  9. Pope C.G. 1948; The assay of penicillin by the dilution method and the differential assay of penicillin by charcoal adsorption. Analyst 73:247
    [Google Scholar]
  10. Wood E.C. 1948; Discussion on methods of penicillin assay. Analyst 73:256
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-6-1-2-14
Loading
/content/journal/micro/10.1099/00221287-6-1-2-14
Loading

Data & Media loading...

Most cited Most Cited RSS feed