1887

Abstract

SUMMARY: The endogenous respiration of vegetative amoebae, harvested during active growth, was similar in both the culture medium and in the high salt medium used to induce the encystation of starved amoebae. The respiration of starved amoebae and early stages of encystation was suppressed in high salt medium and stimulated when returned to the culture medium. A transient increase in respiration was observed early in encystation, while the respiration of later encystation stages decreased to the low level observed in mature cysts.

The respiratory metabolism of mitochondria isolated from encysting amoebae was similar to that of mitochondria from vegetative amoebae while cyst mitochondria exhibited little respiration. The catalase and acid phosphatase activities of amoebae increased during starvation and encystation.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-59-3-351
1969-12-01
2021-08-01
Loading full text...

Full text loading...

/deliver/fulltext/micro/59/3/mic-59-3-351.html?itemId=/content/journal/micro/10.1099/00221287-59-3-351&mimeType=html&fmt=ahah

References

  1. Band R. N. 1959; Nutritional and related biological studies on the free-living soil amoeba, Hart-mannella rhysodes. . J. gen. Microbiol 21:80
    [Google Scholar]
  2. Band R. N. 1962; The amino acid requirements of the soil amoeba, Hartmannella rhysodes Singh.. J. Protozool 9:377
    [Google Scholar]
  3. Band R. N. 1963; Extrinsic requirements for encystation by the soil amoeba, Hartmannella rhysodes. . J. Protozool 10:101
    [Google Scholar]
  4. Band R. N., Irvine B. 1965; The electrokinetic characteristics of some small amoebae.. Expl Cell Res 39:121
    [Google Scholar]
  5. Bauer H. 1967; Ultrastruktur und Zell wand bildung von Acanthamoeba sp.. Vischt. naturf. Ges. Zurich 112:173
    [Google Scholar]
  6. Beers R. F., Sizer I. W. 1952; A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase.. J. biol. Chem 195:133
    [Google Scholar]
  7. Bowers B., Korn E. D. 1968; The fine structure of Acanthamoeba castellanii. I. The trophozoite.. J. Cell Biol 39:95
    [Google Scholar]
  8. Bowers B., Korn E. D. 1969; The fine structure of Acanthamoeba castellanii. II. Encystment.. J. Cell Biol 41:786
    [Google Scholar]
  9. Estabrook R. W. 1967; Mitochondrial respiratory control and the polarographic measurement of ADP:O ratios.. Meth. Enzym 10:41
    [Google Scholar]
  10. Griffiths A. J., Hughes D. E. 1968; Starvation and encystment of a soil amoeba Hartmannella castellanii. . J. Protozool 15:673
    [Google Scholar]
  11. Lasman M. 1967; Observations on acid phosphatase in Mayorella palestinensis. . J. cell. comp. Physiol 69:151
    [Google Scholar]
  12. Miller G. L. 1959; Protein determination for large numbers of samples.. Analyt. Chem 31:964
    [Google Scholar]
  13. Müller M., Møller M. 1967; Peroxisomes in Acanthamoeba sp.. J. Protozool 14:(suppl.)11
    [Google Scholar]
  14. Müller M., Hogg J. F., deDuve C. 1968; Distribution of tricarboxylic acid cycle enzymes and glyoxylate cycle enzymes between mitochondria and peroxisomes in Tetrahymena pyriformis. . J. biol. Chem 243:5385
    [Google Scholar]
  15. Neff R. J., Neff R. H., Taylor R. E. 1958; The nutrition and metabolism of a soil amoeba, Acanthamoeba sp.. Physiol. Zoöl 31:73
    [Google Scholar]
  16. Page F. C. 1967; Re-definition of the genus Acanthamoeba with descriptions of three species.. J. Protozool 14:709
    [Google Scholar]
  17. Reich K. 1948; Studies on the respiration of an amoeba, Mayorella palestinensis. . Physiol. Zoöl 21:390
    [Google Scholar]
  18. Reich K. 1955; The effect of cyanide and azide on the respiration of the amoeba Mayorella palestinensis. . Physiol. Zoöl 28:145
    [Google Scholar]
  19. Schneider W. C., Hogeboom G. H. 1950; Intracellular distribution of enzymes. V. Further studies on the distribution of cytochrome c in rat-liver homogenates.. J. biol. Chem 183:123
    [Google Scholar]
  20. Vickerman K. 1962; Patterns of cellular organization in limax amoebae.. Expl Cell Res 26:497
    [Google Scholar]
  21. Wattiaux R., deDuve C. 1956; Tissue fractionation studies. 7. Release of bound hydrolases by means of Triton X-100.. Biochem. J 63:606
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-59-3-351
Loading
/content/journal/micro/10.1099/00221287-59-3-351
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error