1887

Abstract

SUMMARY: Growth of in a tryptose medium, with lipid pre-extracted, containing charcoal-treated bovine plasma albumin, occurred only after addition of long-chain fatty acids. Of the long-chain fatty acids tested, positional as well as geometrical isomers of octadecenoic acids were effective in growth promotion; isomers were more effective in promoting growth and in lowering osmotic fragility than their corresponding isomers. Tetra-decenoic or hexadecenoic acids did not replace the growth requirement for an octadecenoic acid. Although fatty acids containing cyclopropane-rings promoted growth, a greater toxicity and lower growth response was observed. No fatty acid isomerization by this mycoplasma was detected and the octadecenoic acids added to the growth medium were recovered from membrane polar lipids unchanged. Fatty acid analyses revealed that myristic and palmitic acids were the major saturated fatty acid components of the membrane polar lipids. No differences were found in the fatty acid content or composition of membrane polar lipids from mycoplasmas grown at 25° and 37°. Major differences in the fatty acid composition between glycolipids and phospholipids were not apparent.

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/content/journal/micro/10.1099/00221287-59-3-317
1969-12-01
2022-01-19
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References

  1. Ansell G. B., Hawthorne J. N. 1964 Phospholipids43 New York: Elsevier Publishing Co.;
    [Google Scholar]
  2. Butler M., Knight B. C. J. G. 1960; The survival of washed suspensions of Mycoplasma.. J. gen. Microbiol 22:470
    [Google Scholar]
  3. Chapman D., Owens N. F., Walker D. A. 1966; Physical studies of phospholipids. II. Monolayer studies of some synthetic 2,3-diacyl-DL phosphatidylethanolamines and phosphatidylcholines containing trans double bonds.. Biochim. biophys. Acta 120:148
    [Google Scholar]
  4. Chen F. R. 1967; Removal of fatty acids from serum albumin by charcoal treatment.. J. biol. Chem 242:173
    [Google Scholar]
  5. Fiske C. H., SubbaRow Y. 1925; The colorimetric determination of phosphorus.. J. biol. Chem 66:375
    [Google Scholar]
  6. Folch J., Lees M., Stanley G. H. 1957; A simple method for the isolation and purification of total lipids from animal tissues.. J. biol. Chem 226:497
    [Google Scholar]
  7. Henrikson C. V., Panos C. 1969; Fatty acid composition, distribution and requirements of two nonsterol-requiring mycoplasmas from complex but defatted growth media.. Biochemistry 8:646
    [Google Scholar]
  8. Hofmann K. 1963 Fatty Acid Metabolism in Microorganisms. New York: J. Wiley and Sons.;
    [Google Scholar]
  9. Hofmann K., Panos C. 1954; The biotin-like activity of lactobacillic acid and related compounds.. J. biol. Chem 210:687
    [Google Scholar]
  10. Hofmann K., Henis D. B., Panos C. 1957; Fatty acid interconversions in lactobacilli.. J. biol. Chem 228:349
    [Google Scholar]
  11. Hofmann K., Lucas R. A., Sax S. M. 1952; The chemical nature of the fatty acids of Lactobacillus arabinosus. . J. biol. Chem 195:473
    [Google Scholar]
  12. Kates M. 1966; Biosynthesis of lipids in microorganisms.. A. Rev. Biochem 20:13
    [Google Scholar]
  13. Lands W. E. M. 1965; The effect of double bonds on configuration in lecithin synthesis.. J. Am. Oil Chem. Soc 42:465
    [Google Scholar]
  14. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin reagent.. J. biol. Chem 193:265
    [Google Scholar]
  15. McElhaney R. N., Tourtellotte M. E. 1969; Mycoplasma membrane lipids: Variations in fatty acid composition.. Science, N. Y. 164:433
    [Google Scholar]
  16. O’Brien J. S. 1965; Stability of myelin membrane.. Science, N.Y. 147:1099
    [Google Scholar]
  17. O’Leary W. M. 1962; On the fatty acids of the pleuropneumonia-like organisms.. Biochem. biophys. Res. Commun 8:87
    [Google Scholar]
  18. Panos C., Henrikson C. V. 1968; Resolution of positional isomers of bacterial long chain cyclopropane-ring containing fatty acids by capillary column chromatography.. J. Gas Chromat 6:551
    [Google Scholar]
  19. Panos C., Henrikson C. V. 1969; Fatty acid interconversions in Mycoplasma sp. khs.. Bio-chemistry 8:652
    [Google Scholar]
  20. Panos C., RoTtem S. 1969; Incorporation and elongation of fatty acid isomers by Mycoplasma laidlawii A.. Biochemistry (in the Press).
    [Google Scholar]
  21. Panos C., Cohen M., Fagan G. 1966; Lipid alterations after cell wall inhibition. Fatty acid content of Streptococcus pyogenes and derived L-form.. Biochemistry 5:1461
    [Google Scholar]
  22. Pollack J. D., Tourtellotte M. E. 1967; Synthesis of saturated long chain fatty acids from sodium acetate-1-C14 by mycoplasma.. J. Bact 93:636
    [Google Scholar]
  23. Razin S. 1967; The cell membrane of mycoplasma.. Ann. N.Y. Acad. Sci 143:115
    [Google Scholar]
  24. Razin S., Argaman M. 1963; Lysis of mycoplasma, bacterial protoplasts, spheroplasts and L-forms by various agents.. J. gen. Microbiol 30:155
    [Google Scholar]
  25. Razin S., Rottem S. 1963; Fatty acid requirements of Mycoplasma laidlawii. . J. gen. Microbiol 33:459
    [Google Scholar]
  26. Razin S., Tourtellotte M. E., McElhaney R. N., Pollack J. D. 1966; Influence of lipid components of Mycoplasma laidlawii membranes on osmotic fragility of cells.. J. Bact 91:609
    [Google Scholar]
  27. Rodwell A. W. 1967; The nutrition and metabolism of mycoplasma: progress and problems.. Ann. N.Y. Acad. Sci 143:88
    [Google Scholar]
  28. Rodwell A. W. 1968; Fatty acid composition of mycoplasma lipids: biomembrane with only one fatty acid.. Science, N.Y. 160:1350
    [Google Scholar]
  29. Rodwell A. W., Abbot A. 1961; The function of glycerol, cholesterol and long chain fatty acids in the nutrition of Mycoplasma mycoides. . J. gen. Microbiol 25:201
    [Google Scholar]
  30. Rottem S., Panos C. 1969; The synthesis of long chain fatty acids by a cell-free system from Mycoplasma laidlawii A. . Biochemistry (in the Press).
    [Google Scholar]
  31. Rottem S., Razin S. 1967a; Uptake and utilization of acetate by mycoplasma.. J. gen. Microbiol 48:53
    [Google Scholar]
  32. Rottem S., Razin S. 1967b; Electrophoretic patterns of membrane proteins of mycoplasma.. J. Bact 94:359
    [Google Scholar]
  33. Shaw N., Smith P. F., Koostra W. L. 1968; The lipid composition of Mycoplasma laidlawii strain b.. Biochem. J 107:329
    [Google Scholar]
  34. Smith P. F. 1967; Comparative lipid biochemistry of mycoplasma.. Ann. N.Y. Acad. Sci 143:139
    [Google Scholar]
  35. Smith P. F., Koostra W. L., Henrikson C. V. 1965; Diphosphatidyl glycerol in Mycoplasma laidlawii. . J. Bact 90:282
    [Google Scholar]
  36. Tausig F. 1955 The chemical nature of fatty acids of microbiological origin. Ph.D. dissertation: University of Pittsburgh.;
    [Google Scholar]
  37. Weinbaum G., Panos C. 1966; Fatty acid distribution in normal and filamentous Escherichia coli. . J. Bact 92:1576
    [Google Scholar]
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