1887

Abstract

SUMMARY: The stability of penicillinase messenger RNA has been assessed from the kinetics of enzyme production after inhibition of RNA synthesis with actinomycin or rifampicin. An average messenger half-life of 4·7 min. is indicated, both for induced 749, and the constitutive mutant 749/. Although this value is twice that estimated for average total m-RNA by similar techniques, there is no indication of a ‘long-lived messenger’ as previously implicated in other reports. An attempt was made to estimate the content of penicillinase messenger from data on the level of penicillinase made per bacterium, the polysome size, the messenger half-life and the total level of unstable RNA per bacterium. It is suggested that at the maximum induced rate of enzyme formation there are approximately 110 penicillinase messengers per bacterium, and in the constitutive about twice this number.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-59-2-171
1969-12-01
2019-10-21
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-59-2-171
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error