SUMMARY: With a glucose-limited chemostat culture of 1078 the yield factor, , for glucose increased from 0·3 to 0·12 following an increase in the dilution rate from 0·1 to 0·35 hr. However, even when growing at the faster rate the value of still was extremely small compared to those found with other micro-organisms. This low yield value was not due to an incomplete oxidation of glucose, since most of the glucose carbon utilized (83 to 97%) could be accounted for as carbon dioxide, the percentage conversion depending on the dilution rate. The specific respiration rate ( ) of the growing culture was determined and found to remain nearly constant over the range of dilution rates. However, the value of diminished progressively from 0·12 to 0·03 when the dissolved oxygen concentration in a chemostat culture was increased from 3·3 to 5·7 p.p.m. The yield factor, (evaluated by making certain assumptions) was much smaller than the value of 10 found with other microbial culture, even though the value of increased appreciably with the increase of dilution rate. These results are discussed with reference to some of the known physiological characteristics of .


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