1887

Abstract

SUMMARY: The amidohydrolase formed in the later stages of growth on glucose peptone medium by was purified 60-fold from cell-free extracts. The purified enzyme hydrolysed mono- and di-carboxylic acid amides, benzamide, nicotinamide, pyrazinamide and certain β-hydroxyl substituted fatty acid amides, was inactive on -asparagine and -glutamine and was competitively inhibited by urea and -methylurea. Acetamide and nicotinamide together showed mutual competition.

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/content/journal/micro/10.1099/00221287-59-1-47
1969-11-01
2019-10-19
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-59-1-47
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