1887

Abstract

SUMMARY: Acetohydroxyacid synthetase produced by 176 and a high penicillin-producing mutant have been compared. Both were sensitive to valine as the major feedback inhibitor. Inhibition of the enzyme in 176 appears to be effected through two valine binding sites, but only one of these remains in the high yielding strain and it is non-competitive with respect to pyruvate. The amount of enzyme activity detectable in the high yielding strain is more than twice the level in 176. Control of acetohydroxy-acid synthetase by other related amino acids was shown to be complex and involved sites other than the valine binding site.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-59-1-111
1969-11-01
2022-01-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/59/1/mic-59-1-111.html?itemId=/content/journal/micro/10.1099/00221287-59-1-111&mimeType=html&fmt=ahah

References

  1. Armstrong F. B., Wagner R. P. 1963; Repression of the valine-isoleucine pathway in Salmonella.. Proc. natn. Acad. Sci. U.S.A 49:628
    [Google Scholar]
  2. Freundlich M. R., Burns R. O., Umbarger H. E. 1962; Control of isoleucine, valine and leucine biosynthesis (1) Multivalent repression.. Proc. natn. Acad. Sci. U.S.A 48:1804
    [Google Scholar]
  3. Goulden S. A., Chattaway F. W. 1968; Lysine control of α-Aminoadipate and penicillin synthesis in Penicillium chrysogenum. . Biochem. J 110:5556
    [Google Scholar]
  4. Juni E. 1961; Evidence for a two-site mechanism for decarboxylation of α-keto acids by α-carboxylase.. J. biol. Chem 236:2302
    [Google Scholar]
  5. Krampitz L. O. 1948; Synthesis of acetyllactic acid.. Archs. Biochem. Biophys 17:81
    [Google Scholar]
  6. Leavitt R., Umbarger H. E. 1961; Isoleucine and valine metabolism in Escherichia coli. . J. biol. Chem 236:2486
    [Google Scholar]
  7. Magee P. T., Szulmajster H. 1968; Regulation of isoleucine-valine biosynthesis in Saccharomyces cerevisiae. 3. Properties and regulation of the activity of acetohydroxyacid synthetase.. Eur. J. Biochem 3:507
    [Google Scholar]
  8. de Szulmajster H., Magee P. T. 1968; The regulation of isoleucine-valine biosynthesis in Saccharomyces cerevisiae. I. Threonine deaminase.. Eur. J. Biochem 3:492
    [Google Scholar]
  9. Segel I. H., Johnson M. J. 1961; Accumulation of intracellular inorganic sulphate by Penicillium chrysogenum. . J. Bact 81:91
    [Google Scholar]
  10. Tardrew P. L., Johnson M. J. 1958; Sulphate utilisation by penicillin-producing mutants of Penicillium chrysogenum. . J. Bact. 76:400
    [Google Scholar]
  11. Umbarger H. E. 1956; Evidence for a negative feedback mechanism in the biosynthesis of isoleucine.. Science, N. Y. 123:848
    [Google Scholar]
  12. Umbarger H. E., Brown B. 1957; Threonine deamination in Escherichia coli. II. Evidence for two l-threonine deaminases.. J. Bact 73:105
    [Google Scholar]
  13. Umbarger H. E., Davis B. D. 1962; Pathways of amino acid biosynthesis.. The Bacteria Ed. by Gunsalus I. C., Stanier R. Y. 3167 New York: Academic Press.;
    [Google Scholar]
  14. Webb J. L. 1963 Enzyme and Metabolic Inhibitors 1489 New York and London: Academic Press.;
    [Google Scholar]
  15. Westerfeld W. W. 1945; A colorimetric determination of blood acetoin.. J. biol. Chem 161:495
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-59-1-111
Loading
/content/journal/micro/10.1099/00221287-59-1-111
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error