@article{mbs:/content/journal/micro/10.1099/00221287-58-3-411, author = "RIGHELATO, R. C.", title = "The Distribution of Iron in Iron-deficient Toxin-synthesizing and in Excess-iron Non-toxin-synthesizing Corynebacterium Diphtheriae", journal= "Microbiology", year = "1969", volume = "58", number = "3", pages = "411-419", doi = "https://doi.org/10.1099/00221287-58-3-411", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-58-3-411", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "SUMMARY In the presence of excess iron diphtherial toxin was not produced by Corynebacterium diphtheriae cn2000. 2·8 % of the intracellular iron was present in the soluble fraction of washed bacteria; the remainder was bound on the respiratory particles, 4 % as haeme and the rest as non-haeme iron. Succinate but not NADH reduced the cytochromes and a small proportion of the non-haeme iron. Toxin was synthesized at a high rate by bacteria containing 6·5 % of the iron present in excess-iron bacteria. Under such iron-deficient conditions the iron-containing components of the respiratory particles were present in decreased quantities. NADH oxidation, which proceeded via a soluble flavoprotein, was not affected by iron-deficiency, nor could reduction of non-haeme iron by NADH be demonstrated. The transition between the iron-deficient and the excess-iron states was studied.", }