RT Journal Article SR Electronic(1) A1 RIGHELATO, R. C. A1 Van Hemert, P. A.YR 1969 T1 Growth and Toxin Synthesis in Batch and Chemostat Cultures of Corynebacterium Diphtheriae JF Microbiology, VO 58 IS 3 SP 403 OP 410 DO https://doi.org/10.1099/00221287-58-3-403 PB Microbiology Society, SN 1465-2080, AB SUMMARY Toxin synthesis in restricted-iron batch cultures of Corynebacterium diphtheriae cn2000 (a pw8 s pd strain) was proportional to the bacterial mass above a minimum bacterial concentration. During glucose-limited growth 0·30 g. toxin was released/g. bacterial protein synthesized. In excess of glucose 0·06 g. toxin was released/g. bacterial protein synthesized. Toxin release in glucose-limited continuous cultures followed similar kinetics to the batch cultures. Iron in the restricted-iron medium, 6 μg. atom/l., limited the quantities of iron taken up by the bacteria and of three iron-containing enzymes, but did not restrict bacterial concentration over the range 2·0–4·5 mg. bacterial protein/ml. The addition of 90 μg. atom Fe/l. to a restricted-iron culture rapidly inhibited toxin release., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-58-3-403