1887

Abstract

SUMMARY

Toxin synthesis in restricted-iron batch cultures of 2000 (a 8 strain) was proportional to the bacterial mass above a minimum bacterial concentration. During glucose-limited growth 0·30 g. toxin was released/g. bacterial protein synthesized. In excess of glucose 0·06 g. toxin was released/g. bacterial protein synthesized. Toxin release in glucose-limited continuous cultures followed similar kinetics to the batch cultures.

Iron in the restricted-iron medium, 6 g. atom/l., limited the quantities of iron taken up by the bacteria and of three iron-containing enzymes, but did not restrict bacterial concentration over the range 2·0–4·5 mg. bacterial protein/ml. The addition of 90 g. atom Fe/l. to a restricted-iron culture rapidly inhibited toxin release.

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/content/journal/micro/10.1099/00221287-58-3-403
1969-11-01
2021-08-01
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