SUMMARY: Toxin synthesis in restricted-iron batch cultures of CN2ooo (a pw8 PD strain) was proportional to the bacterial mass above a minimum bacterial concentration. During glucose-limited growth 0.30 g. toxin was released/g. bacterial protein synthesized. In excess of glucose 0.06 g. toxin was released/g. bacterial protein synthesized. Toxin release in glucose-limited continuous cultures followed similar kinetics to the batch cultures.

Iron in the restricted-iron medium, 6 μg. atom/1., limited the quantities of iron taken up by the bacteria and of three iron-containing enzymes, but did not restrict bacterial concentration over the range 2.0-4.5 mg. bacterial protein/ml. The addition of 90 μg. atom Fe/1. to a restricted-iron culture rapidly inhibited toxin release.


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