1887

Abstract

SUMMARY

Proflavine in the post-aerosolization plating medium lowered by over 80% the number of viable / organisms recovered from aerosols of 55% relative humidity (RH) and above, but had no effect on the recovery of viable 2 organisms. Oxygen enhanced the lethal action of semi-dehydration on strain /, held at 50% RH and below, but had less effect on the survival of 2. In addition, / organisms displayed an increased rate of respiration, and discharged 260 m absorbing material, when held at intermediate values of RH. These phenomena were not observed with 2 organisms. Thus / seems to possess an energy consuming mechanism by which it is able to survive semi-dehydration above 55% RH by a discharge of damaged cell components. This mechanism is very slowly destroyed when the organisms are semi-dehydrated in nitrogen but, below 50% RH, it is rapidly inactivated by oxygen. It is suggested that the mechanism is a membrane-bound system, possibly involving the cytochrome chain.

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/content/journal/micro/10.1099/00221287-58-3-317
1969-11-01
2021-07-24
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References

  1. Ashwood-Smith M. J., Bridges B. A. 1966; Ultraviolet mutagenesis in Escherichia coli at low temperatures. Mutation Res 3:135
    [Google Scholar]
  2. Borthwick H. A., Henricks S. B., Parker M. W., Tooke E. H., Toole V. 1952; Reversible photoreaction controlling seed germination. Proc. natn. Acad. SciU.S.A 38662
    [Google Scholar]
  3. Cox C. S. 1967; The aerosol survival of Escherichia coli jepp sprayed from protecting agents into nitrogen atmospheres under changing conditions of relative humidity. J. gen. Microbiol 49:109
    [Google Scholar]
  4. Cox C. S. 1968; The aerosol survival and cause of death of Escherichia coli k12. J. gen. Microbiol 54:169
    [Google Scholar]
  5. DeJong J. C., Winkler K. C. 1968; The inactivation of poliovirus in aerosols. J. Hyg., Camb 66:557
    [Google Scholar]
  6. Hess G. C. 1965; Effects of oxygen on aerosolized Serratia marcescens . Appl. Microbiol 13:781
    [Google Scholar]
  7. Hill R. F. 1965; Ultraviolet-induced lethality and reversion to prototrophy in Escherichia coli strains with normal and reduced dark repair ability. Photochem. Photobiol 4:563
    [Google Scholar]
  8. Kelner A. 1949; Photoreactivation of ultraviolet irradiated E. coli with special reference to the dose reduction principle and to ultraviolet induced mutation. J. Bact 58:511
    [Google Scholar]
  9. Lieb M. 1964; Dark repair of u.v. induction in k12 (λ). Virology 23:381
    [Google Scholar]
  10. Stein W., Meutzner I. 1950; Reaktivierung von UV-inaktivierten Bakterium coli durch warme. Naturwissenschaften 37:169
    [Google Scholar]
  11. Webb S. J. 1960; Factors affecting the viability of air-borne bacteria. III. The role of bonded water and protein structure in the death of air-borne cells. Can. J. Microbiol 6:89
    [Google Scholar]
  12. Webb S.J. 1965 Bound Water in Biological Integrity Springfield, Illinois, U.S.A.: Charles C. Thomas;
    [Google Scholar]
  13. Webb S. J. 1967; The influence of oxygen and inositol on the survival of semidried microorganisms. Can. J. Microbiol 13:733
    [Google Scholar]
  14. Webb S. J., Dumasia M. D. 1967a; The induction of lambda prophages by controlled desiccation. Can. J. Microbiol 13:33
    [Google Scholar]
  15. Webb S. J., Dumasia M. D. 1967b; Bound water, inositol and the induction of lambda prophages by ultraviolet light. Can. J. Microbiol 13:303
    [Google Scholar]
  16. Webb S. J., Tai C. C. 1968; Lethal and mutagenic action of 3200–4000 Å light. Can. J. Microbiol 14:727
    [Google Scholar]
  17. Wells W. F. 1955 Air Hygiene and Air Contagion Boston, Massachusetts: Harvard University Press;
    [Google Scholar]
  18. Witkin E. M. 1963; The effect of acriflavin on photoreversal of lethal and mutagenic damage produced in bacteria by ultraviolet light. Proc. natn. Acad. SciU.S.A 50425
    [Google Scholar]
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