1887

Abstract

SUMMARY

Analyses, tests with isotopic nitrogen and tests for acetylene and isocyanide reduction, using both continuous and batch cultures, were made with seven strains of putative nitrogen-fixing bacteria and three local isolates. Only two (single strains of and ) fixed nitrogen; the active pseudomonad differed in several respects from the organism originally reported. Other Pseudomonas, Nocardia and Azotomonas species and the three local isolates did not fix; some simulated nitrogen fixation in cultural tests most impressively, but proved simply to be very efficient scavengers of traces of fixed nitrogen.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-58-2-277
1969-10-01
2021-08-01
Loading full text...

Full text loading...

/deliver/fulltext/micro/58/2/mic-58-2-277.html?itemId=/content/journal/micro/10.1099/00221287-58-2-277&mimeType=html&fmt=ahah

References

  1. Anderson G. R. 1955; Nitrogen fixation by Pseudomonas-like soil bacteria. J. Bact 70:129
    [Google Scholar]
  2. Baker K. 1968; Low cost continuous culture apparatus. Lab. Pract 17:817
    [Google Scholar]
  3. Belyakov E. V. 1957; A new simple method for the isolation of Azotobacter from soil. Mikro-biologiya 262 Rubentchik L. I. 1963 Azotobacter and its Use in Agriculture Jerusalem, Israel: Program of Scientific Translations;
    [Google Scholar]
  4. Biggins D. R., Postgate J. R. 1969; Nitrogen fixation by cultures and cell-free extracts of Mycobacterium flavum 301. J. gen. Microbiol 56:181
    [Google Scholar]
  5. Campbell N. E. R., Dular R., Lees H., Standing K. G. 1967; The production of 13N2 by 50-MeV protons for use in biological nitrogen fixation. Can. J. Microbiol 13:587
    [Google Scholar]
  6. Dalton H., Postgate J. R. 1969; Effect of oxygen on growth of Azotobacter chroococcum in batch and continuous culture. J. gen. Microbiol 54:463
    [Google Scholar]
  7. De Ley J. 1968; DNA base composition and classification of some more free-living nitrogen-fixing bacteria. Antonie van Leeuwenhoek 34:66
    [Google Scholar]
  8. De Ley J., Park I. W. 1966; Molecular biological taxonomy of some free-living nitrogen-fixing bacteria. Antonie van Leeuwenhoek 32:6
    [Google Scholar]
  9. Dilworth M. J. 1966; Acetylene reduction by nitrogen-fixing preparations from Cl. pasteurianum . Biochim. biophys. Acta 127:285
    [Google Scholar]
  10. Fedorov M. V., Kalininskaya T. A. 1961; Anew species of nitrogen-fixing Mycobacterium and its physiological properties. Mikrobiologiya 30:7
    [Google Scholar]
  11. Hino S., Wilson P. W. 1958; Nitrogen fixation by a facultative Bacillus. J. Bact 15:403
    [Google Scholar]
  12. Jensen H. L., Petersen E. J., De P. K., Bhattacharaya R. 1960; A new-nitrogen-fixing bacterium: Derxia gummosa nov. gen. nov. spec. Arch. Mikrobiol 36:182
    [Google Scholar]
  13. Kelly M. 1968; The kinetics of the reduction of isocyanides, acetylenes and the cyanide ion by nitrogenase preparations from Azotobacter chroococcum and the effects of inhibitors. Biochem. J 107:1
    [Google Scholar]
  14. Krasil’nikov N. A. 1945; A new species of heterotrophic nitrogen-fixer, Azotomonas fluorescens . Ref. nauchno-issled. Rab. Akad. Nauk SSSR L’vov N. P. 1963
    [Google Scholar]
  15. L’vov N. P. 1963; New free-living nitrogen-fixing micro-organisms. Izv. Akad. Nauk SSSR Ser. biol 2:270
    [Google Scholar]
  16. Metcalfe G., Brown M. E. 1957; Nitrogen Fixation by a new species of Nocardia. J. gen. Microbiol 17:567
    [Google Scholar]
  17. Meynell G. G., Meynell E. 1965 Theory and Practice in Experimental Bacteriology, 1st.4 Cambridge: Microbiology Society;
    [Google Scholar]
  18. Newman A. C. D. 1966; A distillation of ammonia for isotopic analysis. Chemy Ind3115
    [Google Scholar]
  19. Newton J. W., Wilson P. W., Burris R. H. 1953; Direct determination of ammonia as an intermediate in nitrogen fixation by Azotobacter. J. biol. Chem 204:445
    [Google Scholar]
  20. Nicholas D. J. D. 1963; The biochemistry of nitrogen-fixation. Symp. Soc. gen. Microbiol 13:92
    [Google Scholar]
  21. Parejko R. A., Wilson P. W. 1968; Taxonomy of Azotomonas species. J. Bact 95:143
    [Google Scholar]
  22. Paul E. A., Newton J. D. 1961; Studies of aerobic non-symbiotic nitrogen-fixing bacteria. Can. J. Microbiol 7:7
    [Google Scholar]
  23. Postgate J. R. 1967; Soil bacteria parasitic on Azotobacteriaceae. Antonie van Leeuwenhoek 33:113
    [Google Scholar]
  24. Schöllhorn R., Burris R. H. 1967; Reduction of azide by the N2-fixing enzyme system. Proc. natn. Acad. SciU.S.A 571317
    [Google Scholar]
  25. Sprinson D. B., Rittenberg D. 1949; The rate of utilization of ammonia from protein synthesis. J. biol. Chem 180:707
    [Google Scholar]
  26. Stapp C. 1940; Azotomonas insolita ein neuer aeroberstickstoffbindender Mikroorganismus. Zentbl. Bakt. Parasit Kde (Abt II) 102:1
    [Google Scholar]
  27. Stewart W. D. P. 1966 Nitrogen Fixation in Plants, 1st.76 London: Athlone Press;
    [Google Scholar]
  28. Stickland L. H. 1951; The determination of small quantities of bacteria by means of the biuret reaction. J. gen. Microbiol 5:698
    [Google Scholar]
  29. Stockdale H., Ribbons D. W., Dawes E. A. 1968; Occurrence of poly-β-hydroxybutrate in the Azotobacteriaceae. J. Bact 95:1798
    [Google Scholar]
  30. Voets J. P., Debacher J. 1956; P. azotogensis nov. spp. A new free-living nitrogen-fixing bacterium. Naturwissenschaften 43:40
    [Google Scholar]
  31. Wilson P. W. 1952; The comparative biochemistry of nitrogen fixation. Adv. Enzymol 13:345
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-58-2-277
Loading
/content/journal/micro/10.1099/00221287-58-2-277
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error