1887

Abstract

SUMMARY

Five mutations of 2 have been mapped by transduction. They were isolated from five motile ( ) strains each with a different form of antigen-, caused by mutation of , the structural gene for phase-1 flagellin. was cotransduced (frequency 0·1 to 0·5) with each of the mutations. Three-point crosses using a serological selection technique indicate the following order: -50—-58—-55- The position of -52 was not discovered. If the complementation groups correspond to the genes their order is .

Of the five mutations four (M5, M20, M11, M12) appear to be very closely linked. The fifth (M6) is outside this cluster. Transductants with functionally and antigenically normal antigen- were obtained, at a very low frequency, in crosses between -curly and strains: they are attributed to crossing over within between the ‘curly’ mutation and the mutations causing alteration in serological character. Some earlier conclusions (Joys & Stocker, 1963) as to gene order, especially within , now seem unjustified.

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/content/journal/micro/10.1099/00221287-58-2-267
1969-10-01
2022-01-27
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