The lactic dehydrogenases of 11 strains of the genus Leuconostoc were examined. All possessed a d(–) but no l(+) NAD-dependent lactic dehydro-genase, and three strains (1 L. mesenteroides and 2 L. paramesenteroides) also had d(–) and l(+) NAD-independent lactic dehydrogenases. The NAD-dependent enzyme of the seven strains belonging to the species L. mesenteroides, L. dextranicum, L. paramesenteroides, L. lactis and L. cremoris moved together during electrophoresis in acrylamide gel. The enzymes of the other four strains, all L. oenos, were close together but widely separated from the enzyme of the other species. One strain of L. mesenteroides oxidized NADH without added pyruvate. The enzyme responsible was located after electro-phoresis.
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