1887

Abstract

SUMMARY

Mycoplasma strain , serologically and biochemically related to grew well in a medium containing unesterified fatty acids, cholesterol and some samples of fatty acid-poor bovine serum albumin. With other samples of serum albumin, growth was accompanied by cellular lysis. Heating albumin solutions, treating them with charcoal, or varying the ratio of fatty acids to albumin, the concentration of cholesterol, or the order in which the lipid components were added to the medium, all influenced the amount of lysis. These effects may be due to varying interaction between fatty acids and cholesterol and between fatty acids and albumin. When fatty acids are firmly bound to albumin, their interaction with cholesterol is prevented, resulting in a cholesterol deficiency. Lysis was prevented by the addition of a heat-stable defatted serum protein fraction (fraction C), or a pronase digest of fraction C, both of which dispersed cholesterol.

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/content/journal/micro/10.1099/00221287-58-1-29
1969-09-01
2024-04-19
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References

  1. Bartlett G. R. 1959; Phosphorus assay in column chromatography. J. biol. Chem 234:466
    [Google Scholar]
  2. Bray G. A. 1960; A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter. Analyt. Biochem 1:279
    [Google Scholar]
  3. Byfield J. E., Scherbaum O. H. 1966; A rapid radioassay technique for cellular suspensions. Analyt. Biochem 17:434
    [Google Scholar]
  4. Chen R. F. 1967; Removal of fatty acids from serum albumin by charcoal treatment. J. biol. Chem 242:173
    [Google Scholar]
  5. Folch J., Lees M., Sloane Stanley G. H. 1957; A simple method for the isolation and purification of total lipids from animal tissues. J. biol. Chem 226:497
    [Google Scholar]
  6. Goodman D. S. 1957; Preparation of human serum albumin free of long-chain fatty acids. Science, N.Y 125:1296
    [Google Scholar]
  7. Kessler J. I., Demeny M., Sobotka H. 1967; Rates of tissue uptake of palmitic acid-1-14C complexed with albumin by two different procedures. J. Lipid Res 8:185
    [Google Scholar]
  8. Laws L. 1956; A pleuropneumonia-like organism causing peritonitis in goats. Aust. vet. J 32:326
    [Google Scholar]
  9. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. J. biol. Chem 193:265
    [Google Scholar]
  10. Marinetti G. V. 1962; Chromatographic separation, identification, and analysis of phosphatides. J. Lipid Res 3:1
    [Google Scholar]
  11. Rodwell A. W. 1956; The role of serum in the nutrition of Asterococcus mycoides . Aust. J. biol. Sci 9:105
    [Google Scholar]
  12. Rodwell A. W. 1960; Nutrition and metabolism of Mycoplasma mycoides var. mycoides . Ann. N.Y. Acad. Sci 79:499
    [Google Scholar]
  13. Rodwell A. W. 1967; The nutrition and metabolism of Mycoplasma: progress and problems. Ann. N.Y. Acad. Sci 143:88
    [Google Scholar]
  14. Rodwell A. W. 1968; Fatty acid composition of Mycoplasma lipids: biomembrane with only one fatty acid. Science, N.Y 160:1350
    [Google Scholar]
  15. Rodwell A. W., Abbot A. 1961; The function of glycerol, cholesterol and long-chain fatty acids in the nutrition of Mycoplasma mycoides . J. gen. Microbiol 25:201
    [Google Scholar]
  16. Smith P. F., Boughton J. E. 1960; Role of protein and phospholipid in the growth of pleuropneumonia-like organisms. J. Bact 80:851
    [Google Scholar]
  17. Smith P. F., Lecce J. G., Lynn R. J. 1954; A lipoprotein as a growth factor for certain pleuropneumonia-like organisms. J. Bact 68:627
    [Google Scholar]
  18. Sodhi H. S., Gould R. G. 1967; Combination of delipidized high density lipoprotein with lipids. J. biol. Chem 242:1205
    [Google Scholar]
  19. Turner A. W., Campbell A. D., Dick A. T. 1935; Recent work on pleuropneumonia Contagiosa bourn in North Queensland. Aust. vet. J 11:63
    [Google Scholar]
  20. Wycoff H. D., Parsons J. 1957; Chromatographic micro-assay for cholesterol and cholesterol esters. Science, N.Y 125:347
    [Google Scholar]
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