1887

Abstract

SUMMARY

Several mutants isolated from three strains showed characteristics differentiating them from the wild type and also from the normal type of non-mucoid mutants. These mutants synthesize much less polysaccharide slime or capsule than the parent bacteria at low incubation temperature, but similar amounts at 37°. The colonies are of rough appearance at 20° and liquid cultures at this temperature autoagglutinate. At 37°, mutant and parental types are indistinguishable. The mutants show altered phage sensitivity patterns at 20° and the yield of lipopolysaccharide appears to be decreased at lower temperatures. Double mutants retaining certain of these characteristics but devoid of exopolysaccharide-synthesizing activity have also been isolated. The properties of the parent and mutant types are compared.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-57-3-369
1969-08-01
2021-10-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/57/3/mic-57-3-369.html?itemId=/content/journal/micro/10.1099/00221287-57-3-369&mimeType=html&fmt=ahah

References

  1. Beiser S. M., Davis B. D. 1957; Mucoid mutants of Escherichia coli. J. Bact 74:303
    [Google Scholar]
  2. Davis B. D., Mingioli E. D. 1950; Mutants of Escherichia coli, requiring methionine or vitamin B12 . J. Bact 60:17
    [Google Scholar]
  3. Dische Z., Subtitles L. B., Osnos M. 1949; New specific colour reactions of hexoses and spectro-photometric micromethods for their determination. Archs Biochem. Biophys 22:169
    [Google Scholar]
  4. Dudman W. F., Wilkinson J. F. 1956; The composition of the extracellular polysaccharide of Aerobacter-Klebsiella strains. Biochem. J 62:289
    [Google Scholar]
  5. Feather M. S., Whistler R. L. 1962; Isolation and characterisation of the principle hemicellulose from corn germ. Archs. Biochem. Biophys 98:111
    [Google Scholar]
  6. Fischer F. G., Dörfel H. 1955; Die papier-chromatographische Trennung und Bestimmung der Uronsauren. Hoppe-Seyler’s Z. physiol. Chem 301:224
    [Google Scholar]
  7. Gahan L. C., Conrad H. E. 1968; An enzyme system for de novo biosynthesis of glycogen in Aerobacter aerogenes . Biochemistry, N. Y 7:3979
    [Google Scholar]
  8. Hestrin S. 1949; The reaction of acetylcholine and other carboxylic acid derivatives with hydroxyl-amine and its analytical application. J. biol. Chem 180:249
    [Google Scholar]
  9. Knolle P., Ørskov I. 1967; The identity of the f+ antigen and the cellular receptor for the RNA phage fr. Molec. Gen. Genetics 99:109
    [Google Scholar]
  10. Koeltzow D. E., Epley J. D., Conrad H. E. 1968; The lipopolysaccharides of Aerobacter aerogeones strains a3(sl) and nctc 243. Biochemistry, N.Y 7:2920
    [Google Scholar]
  11. Lüderrrz O., Staub A. M., Westphal O. 1966; Immunochemistry of O and R antigens of Salmonella and related Enterobacteriaceae. Bact. Rev 30:192
    [Google Scholar]
  12. Lüderttz O., Risse H. J., Shulte-Holthausen H., Strominger J. L., Sutherland I. W., Westphal O. 1965; Biochemical studies of the smooth-rough mutation in Salmonella minnesota . J. Bact 89:343
    [Google Scholar]
  13. McPhee D. G., Sutherland I. W., Wilkinson J. F. 1969; Transduction in Klebsiella. Nature,Lord 221:475
    [Google Scholar]
  14. Smith E. E., Mills G. T., Harper E. M. 1957; A comparison of the uridine pyrophospho-glycosyl metabolism of capsulated and non-capsulated pneumococci. J. gen. Microbiol 16:426
    [Google Scholar]
  15. Sutherland I. W. 1967; Phage-induced fucosidases hydrolysing the exopolysaccharide of Klebsiella aerogenes type 54 [a3(sl)]. Biochem. J 104:278
    [Google Scholar]
  16. Sutherland I. W., Wilkinson J. F. 1965; Depolymerases for bacterial exopolysaccharides obtained from phage-infected bacteria. J. gen. Microbiol 39:383
    [Google Scholar]
  17. Sutherland I. W., Wilkinson J. F. 1966; The composition of lipopolysaccharides of Klebsiella aerogenes and Aerobacter cloacae . Biochim. biophys. Acta 117:261
    [Google Scholar]
  18. Sutherland I. W., Wilkinson J. F. 1968; The exopolysaccharide of Klebsiella aerogenes a3(sl) (type 54). Biochem. J. no749
    [Google Scholar]
  19. Wilkinson J. F., Dudman W. F., Aspinall G. O. 1955; The extracellular polysaccharide of Aerobacter aerogenes a3(sl) (Klebsiella type 54). Biochem. J 59:446
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-57-3-369
Loading
/content/journal/micro/10.1099/00221287-57-3-369
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error