1887

Abstract

SUMMARY

3-Hydroxybutyrate dehydrogenase was studied in 14 Rhizobium strains representing six species. Cell-free extracts of the bacteria were subjected to electrophoresis in starch or polyacrylamide gels and 3-hydroxybutyrate dehydrogenase was located on the gels. Also, the rate of reaction of the enzyme, from different Rhizobium strains, with NAD was compared with its rate of reaction with various analogues of NAD. Most of the Rhizobium strains had characteristic 3-hydroxybutyrate dehydrogenase patterns on gel electropherograms and these patterns were highly reproducible. Multiple forms of 3-hydroxybutyrate dehydrogenase were detected in many of the Rhizobium strains.

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/content/journal/micro/10.1099/00221287-57-3-287
1969-08-01
2024-12-13
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References

  1. Allen O. N. 1957 Experiments in Soil Bacteriology, 3. Minneapolis: Burgess Publishing Co.;
    [Google Scholar]
  2. Bergersen F. J. 1960; Incorporation of l5N2 into various fractions of soybean root nodules. J. gen. Microbiol 22:671
    [Google Scholar]
  3. Bergmeyer H. U. 1965 Methods of Enzymatic Analysis Bergmeyer H. U. London: Academic Press.;
    [Google Scholar]
  4. Bergmeyer H. U., Gawehn K., Klotzsch H., Krebs H. A., Williamson D. H. 1967; Purification and properties of crystalline 3-hydroxybutyrate dehydrogenase from Rhodopseudomonas spheroides. Biochem. J 102:423
    [Google Scholar]
  5. Cann D. C., Wnxox M. E. 1965; Analysis of multimolecular enzymes as an aid to the identification of certain rapidly growing mycobacteria, using starch gel electrophoresis. J. appl. Bact 28:165
    [Google Scholar]
  6. Carr N. G. 1966; The occurrence of poly-β-hydroxybutyrate in the blue-green alga Chlorogloea fritschii. Biochim. biophys. Acta 120:308
    [Google Scholar]
  7. Davis B. J. 1964; Disc electrophoresis. II. Method and application to human serum proteins. Ann.N.Y. Acad. Sci 121:404
    [Google Scholar]
  8. Dawes E. A., Ribbons D. W. 1964; Some aspects of the endogenous metabolism of bacteria. Bact. Rev 28:126
    [Google Scholar]
  9. Fottrell P. F. 1966; Dehydrogenase isoenzymes from legume root nodules.. Nature, Lond 210:198
    [Google Scholar]
  10. Gornall A. G., Bardawill C. J., David M. M. 1949; Determination of serum proteins by means of the biuret reaction. J. biol. Chem 177:751
    [Google Scholar]
  11. Graham P. H. 1963; Antigenic affinities of the root-nodule bacteria of legumes. Antonie van Leeuwenhoek 32:410
    [Google Scholar]
  12. Graham P. H. 1964; The application of computer techniques to the taxonomy of the root-nodule bacteria of legumes. J. gen. Microbiol 35:511
    [Google Scholar]
  13. Green D. E., Dawan J. G., Leloir L. F. 1937; The β-hydroxybutyric dehydrogenase of animal tissues. Biochem. J 31:934
    [Google Scholar]
  14. Hayward A. C, Forsyth W. G. C., Roberts J. B. 1959; Synthesis and breakdown of poly-β-hydroxybutyric acid by bacteria. J. gen. Microbiol 20:510
    [Google Scholar]
  15. Heberlein G. T., De Ley J., Tijtgat R. 1967; Deoxyribonucleic acid homology and taxonomy of Agrobacterium, Rhizobium and Chromobacterium . J. Bact 94:116
    [Google Scholar]
  16. Heidrich H. G. 1968; New aspects on the heterogeneity of beef liver catalase. Hoppe-Seyler’s Z.physiol. Chem 349:873
    [Google Scholar]
  17. van der Helm H. J. 1962; Isoenzymes in different parts of the brain. J. Neurochem 9:325
    [Google Scholar]
  18. Kaplan N. O. 1963; Multiple forms of enzymes. Bact. Rev 27:155
    [Google Scholar]
  19. Kaplan N. O. 1968; Nature of multiple molecular forms of enzymes. Ann. N.Y. Acad. Sci 151:382
    [Google Scholar]
  20. Kaplan N. O., Ciotti M. M. 1961; Evolution and differentiation of dehydrogenases. Ann. N.Y.Acad. Sci 94:701
    [Google Scholar]
  21. Klucas R. V., Evans H. J. 1968; An electron donor system for nitrogenase dependent acetylene reduction by extracts of soybean nodules. Pl. Physiol. Lancaster 43:1458
    [Google Scholar]
  22. ’tMannetje M. 1967; A re-examination of the taxonomy of the genus Rhizobium and related genera using numerical analysis. Antonie van Leeuwenhoek 33:477
    [Google Scholar]
  23. O’Hora A., Fottrell P. F. 1968; Isoenzymes of beta-hydroxybutyrate dehydrogenase in Rhizobium spp. Biochem. J. no16
    [Google Scholar]
  24. Peberdy J. F., Turner M. 1968; The esterases of Mortierella ramanniana in relation to taxonomy. J. gen. Microbiol 51:303
    [Google Scholar]
  25. Shuster C. W., Doudoroff M. 1962; A cold-sensitive d-(β)-hydroxybutyric acid dehydrogenase from Rhodospirillum rubrum . J. biol. Chem 237:603
    [Google Scholar]
  26. Smithies O. 1959; An improved procedure for starch gel electrophoresis: further variations in the serum proteins of normal individuals. Biochem. J 71:585
    [Google Scholar]
  27. Tatt A. 1968; Genetic control of β-hydroxybutyrate dehydrogenase in Paramecium aurelia . Nature,Lond 2x9:941
    [Google Scholar]
  28. Williams R. A. D., Bowden E. 1968; The starch-gel electrophoresis of glucose-6-phosphate dehydrogenase and glyceraldehyde-3-phosphate dehydrogenase of Streptococcus faecalis, S. faecium and S. durans. J. gen. Microbiol 50:329
    [Google Scholar]
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