1887

Abstract

SUMMARY

Mutants of 8602 were isolated which, unlike the wild type, were able to grow with butyramide as a carbon source. Six mutants derived from the constitutive strain 11 were shown to produce an enzyme (B amidase) with altered electrophoretic mobility and altered substrate specificity. The apparent for butyramide of the B amidase was about a tenth of that of the A amidase and the was about ten-fold greater. A further mutation produced mutants able to grow on valeramide.

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/content/journal/micro/10.1099/00221287-57-2-273
1969-08-01
2021-10-28
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References

  1. Brammar W. J., Clarke P. H. 1964; Induction and repression of Pseudomonas aeruginosa amidase. J. gen. Microbiol 37:307
    [Google Scholar]
  2. Brammar W. J., Clarke P. H., Skinner A. J. 1967; Biochemical and genetic studies with regulator mutants of the Pseudomonas aeruginosa 8602 amidase system. J. gen. Microbiol 47:87
    [Google Scholar]
  3. Brammar W. J., McFarlane N. D., Clarke P. H. 1966; The uptake of aliphatic amides by Pseudomonas aeruginosa. J. gen. Microbiol 44:303
    [Google Scholar]
  4. Brown J. E. 1969 Regulator and structural gene mutants of Pseudomonas aeruginosa amidase. Ph.D. Thesis University of London;
    [Google Scholar]
  5. Davis B. J. 1964; Disc electrophoresis-II. Method and application to human serum proteins. Ann. N. Y. Acad. Sci 121:404
    [Google Scholar]
  6. Demerec M., Adelberg E. A., Clark A. J., Hartman P. E. 1966; A proposal for a uniform nomenclature in bacterial genetics. Genetics, Princeton 54:61
    [Google Scholar]
  7. Gammack D. B., Huehns E. R., Shooter E. H., Gerald P. 1960; Identification of the abnormal polypeptide chain of haemoglobin G1b . J. molec. Biol 2:372
    [Google Scholar]
  8. Kelly M., Clarke P. H. 1962; An inducible amidase produced by a strain of Pseudomonas aeruginosa. J. gen. Microbiol 27:305
    [Google Scholar]
  9. Kelly M., Kornberg H. L. 1964; Purification and properties of acyltransferases from Pseudomonas aeruginosa. Biochem. J 93:557
    [Google Scholar]
  10. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. J. biol. Chem 193:265
    [Google Scholar]
  11. McFarlane N. D., Brammar W. J., Clarke P. H. 1965; Esterase activity of Pseudomonas aeruginosa amidase. Biochem. J 95:24C
    [Google Scholar]
  12. Pollock M. R. 1965; Purification and properties of penicillinases from two strains of Bacillus licheniformis: A chemical, physiochemical and physiological comparison. Biochem. J 94:666
    [Google Scholar]
  13. Skinner A. J., Clarke P. H. 1968; Acetate and acetamide mutants of Pseudomonas aeruginosa 8602. J. gen. Microbiol 50:183
    [Google Scholar]
  14. Wu T. T., Lin E. C. C., Tanaka S. 1968; Mutants of Aerobacter aerogenes capable of utilizing xylitol as a novel carbon source. J. Bact 86:447
    [Google Scholar]
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