1887

Abstract

SUMMARY

The growth of some strains of on solid medium was inhibited by rabbit antisera incorporated in filter-paper discs. Other strains were resistant to the same antisera. Rings of precipitate were observed in the agar around the discs. The largest number of precipitation rings and the most intense ones occurred with antiserum-sensitive strains. The antiserum-sensitive strains were also more sensitive than the resistant strains to colistin and polymyxin B when these antibiotics were incorporated in paper discs. Colonies of antiserum-resistant strains developed on solid medium containing low concentrations of horse serum, while colonies of sensitive strains developed only on solid medium containing higher concentrations of serum. The growth of antiserum-sensitive strains, but not resistant ones, was suppressed on solid medium at pH 6·5. All these differences between sensitive and resistant strains were only expressed on solid medium; the phenomena were not observed when the organisms were grown in liquid medium. It is suggested that the differences between sensitive and resistant strains are due to changes in the composition of the mycoplasma membranes.

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/content/journal/micro/10.1099/00221287-57-2-263
1969-08-01
2024-12-09
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References

  1. Clyde W. A., Jun. J. 1964; Mycoplasma species identification based upon growth inhibition by specific antisera. J. Immun 92:958
    [Google Scholar]
  2. Van Deenan L. K. M. 1965; Phospholipids and biomembranes. In Progress in the Chemistry of Fats and other Lipids Holman R. T. 8 Oxford: Pergamon Press;
    [Google Scholar]
  3. Dinter Z., Danielsson D., Bakos K. 1965; Differentiation of porcine mycoplasma strains. J. gen. Microbiol 41:77
    [Google Scholar]
  4. Hayflick L. 1965; Tissue cultures and mycoplasmas. Tex. Rep. Biol. Med Suppl. 1 23:285
    [Google Scholar]
  5. Hayflick L., Stanbridge E. 1967; Isolation and identification of mycoplasma from human clinical materials. Ann. N. Y. Acad. Sci 143:608
    [Google Scholar]
  6. Newton B. A. 1956; The properties and mode of action of the polymyxins. Bact. Rev 20:14
    [Google Scholar]
  7. Razin S., Cosenza B. J., Tourtellotte M. E. 1967; Filamentous growth of mycoplasma. Ann. N.Y.Acad. Sci 143:66
    [Google Scholar]
  8. Sebek O. K. 1967; Polymyxins and circulin. In Antibiotics Gottlieb D., and Shaw P. D. 1142 Berlin-Heidelberg-New York: Springer-Verlag;
    [Google Scholar]
  9. Sobeslavsky O., Prescott B., James W. D., Chanock R. M. 1966; Isolation and characterization of fractions of Mycoplasma pneumoniae. II. Antigenicity and immunogenicity. J. Bact 91:2126
    [Google Scholar]
  10. Switzer W. P. 1955; Studies on infectious atrophic rhinitis. IV. Characterization of a pleuro- pneumonia-like organism isolated from the nasal cavities of swine. Am. J. vet. Res 16:540
    [Google Scholar]
  11. Taylor-Robinson D., Dinter Z. 1968; Unexpected serotypes of mycoplasmas isolated from pigs. J. gen. Microbiol 53:221
    [Google Scholar]
  12. Taylor-Robinson D., Purcell R. H., Wong D. C., Chanock R. M. 1966; A colour test for the measurement of antibody to certain mycoplasma species based upon the inhibition of acid production. J. Hyg., Camb 64:91
    [Google Scholar]
  13. Williams M. H., Taylor-Robinson D. 1967; Antigenicity of mycoplasma membranes. Nature, Lond 215:973
    [Google Scholar]
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