The lysis of Escherichia coli following exposure to EDTA in tris buffer is similar for most F+, F– and Hfr strains. Survival and release of acid soluble nucleotide material was identical in male and female strains. Following osmotic shock by the EDTA + tris method, regrowth patterns of F–, F+ and Hfr strains showed no significant differences. Physiological buffers—Tes, Hepes, Bicine—cannot replace tris in EDTA + lysozyme lysis of E. coli or in osmotic shock to release surface enzymes. Tes, Hepes and Bicine can replace tris in causing lysis of Pseudomonas aeruginosa either with EDTA alone or with lysozyme.
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