SUMMARY: The lysis of following exposure to EDTA in tris buffer is similar for most F, F and Hfr strains. Survival and release of acid soluble nucleotide material was identical in male and female strains. Following osmotic shock by the EDTA + tris method, regrowth patterns of F, F and Hfr strains showed no significant differences. Physiological buffers—Tes, Hepes, Bicine—cannot replace tris in EDTA + lysozyme lysis of or in osmotic shock to release surface enzymes. Tes, Hepes and Bicine can replace tris in causing lysis of either with EDTA alone or with lysozyme.


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