1887

Abstract

SUMMARY

The lysis of following exposure to EDTA in tris buffer is similar for most F, F and Hfr strains. Survival and release of acid soluble nucleotide material was identical in male and female strains. Following osmotic shock by the EDTA + tris method, regrowth patterns of F, F and Hfr strains showed no significant differences. Physiological buffers—Tes, Hepes, Bicine—cannot replace tris in EDTA + lysozyme lysis of or in osmotic shock to release surface enzymes. Tes, Hepes and Bicine can replace tris in causing lysis of either with EDTA alone or with lysozyme.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-57-2-215
1969-08-01
2021-10-27
Loading full text...

Full text loading...

/deliver/fulltext/micro/57/2/mic-57-2-215.html?itemId=/content/journal/micro/10.1099/00221287-57-2-215&mimeType=html&fmt=ahah

References

  1. Goldschmidt M. C, Goldschmidt E. P., Wyss O. 1967; Differences in toxicity of EDTA and tris among mating types of Escherichia coli . Can. J. Microbiol 13:1401
    [Google Scholar]
  2. Good N. E., Winget G. D., Winter W., Connolly T. N., Izawa S., Singh R. M. M. 1966; Hydrogen ion buffers for biological research. Biochemistry, N. Y 5:467
    [Google Scholar]
  3. Gray G. W., Wilkinson S. G. 1965; The effect of ethylenediaminetetra-acetic acid on the cell walls of some Gram-negative bacteria. J. gen. Microbiol 39:385
    [Google Scholar]
  4. Leive L. 1965; A non-specific increase in permeability in Escherichia coli produced by EDTA. Proc. natn. Acad. SciU.S.A 53745
    [Google Scholar]
  5. Neu H. C. 1967; The 5′-nucleotidase of E. coli. I. Purification and properties. J.biol. Chem 242:3896
    [Google Scholar]
  6. Neu H. C., Chou J. 1967; Release of surface enzymes in Enterobacteriaceae by osmotic shock. J. Bact 94:1934
    [Google Scholar]
  7. Neu H. C., Heppel L. A. 1964; On the surface localization of enzymes in Escherichia coli . Biochem. biophys. Res. Commun 17:215
    [Google Scholar]
  8. Neu H. C, Ashman D. F., Price T. D. 1966; The release of acid-soluble nucleotide pool of E. coli by EDTA-Tris. Biochem. biophys. Res. Commun 25:615
    [Google Scholar]
  9. Neu H. C, Ashman D. F., Price T. D. 1967; Effect of ethylenediaminetetra-acetic acid-tris (hydroxymethyl) aminomethane on release of the acid-soluble nucleotide pool and on breakdown of ribosomal ribonucleic acid. J. Bact 93:1360
    [Google Scholar]
  10. Repaske R. 1958; Lysis of Gram-negative organisms and the role of versene. Biochem. biophys. Acta 30:225
    [Google Scholar]
  11. Voss J. G. 1967; Effects of organic cations on the Gram-negative cell wall and their bacteriocidal activity with ethylenediaminetetra-acetate and surface active agents. J. gen. Microbiol 48:391
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-57-2-215
Loading
/content/journal/micro/10.1099/00221287-57-2-215
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error