@article{mbs:/content/journal/micro/10.1099/00221287-57-1-81, author = "COLEMAN, G. S.", title = "The Cultivation of the Rumen Ciliate Entodinium simplex", journal= "Microbiology", year = "1969", volume = "57", number = "1", pages = "81-90", doi = "https://doi.org/10.1099/00221287-57-1-81", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-57-1-81", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "SUMMARY Entodinium simplex has been maintained in vitro anaerobically and in the presence of bacteria in a salts medium containing autoclaved rumen fluid, wholemeal flour and dried grass. The protozoa divided at least every 3 days during more than 2½ years. The wholemeal flour could be replaced by a mixture of rice starch and washed bran but not by either separately; in the presence of this mixture population densities of over 60,000 protozoa/ml. were obtained. Omission of the autoclaved rumen fluid decreased the number of protozoa to 25 % of this value. Evidence was obtained that it was the particulate matter in the rumen fluid that was an essential for growth.", }