1887

Abstract

SUMMARY

has been maintained anaerobically and in the presence of bacteria in a salts medium containing autoclaved rumen fluid, wholemeal flour and dried grass. The protozoa divided at least every 3 days during more than 2½ years. The wholemeal flour could be replaced by a mixture of rice starch and washed bran but not by either separately; in the presence of this mixture population densities of over 60,000 protozoa/ml. were obtained. Omission of the autoclaved rumen fluid decreased the number of protozoa to 25 % of this value. Evidence was obtained that it was the particulate matter in the rumen fluid that was an essential for growth.

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1969-07-01
2022-05-29
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