SUMMARY: has been maintained anaerobically and in the presence of bacteria in a salts medium containing autoclaved rumen fluid, wholemeal flour and dried grass. The protozoa divided at least every 3 days during more than 2½ years. The wholemeal flour could be replaced by a mixture of rice starch and washed bran but not by either separately; in the presence of this mixture population densities of over 60,000 protozoa/ml. were obtained. Omission of the autoclaved rumen fluid decreased the number of protozoa to 25% of this value. Evidence was obtained that it was the particulate matter in the rumen fluid that was an essential for growth.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error