1887

Abstract

SUMMARY

The electrophoretic mobilities of conidia of and , basidiospores of , sporangia and encysted zoospores of were determined in solution at various pH values. The spores all had characteristic and distinct pH-mobility curves. The zero mobility of sporangia over the range pH 2 to 11 is consistent with a cellulose surface free from ionizable groups. The mobility of basidiospores of depended entirely on the presence of carboxyl groups. Chemical and enzymic treatments showed both amino and carboxyl groups on and ; phosphate was present in addition on . The amino groups of -lysine, histidine and leucine contributed to the surface charge of ; amino acids and tyrosine were detected on . The surface of was protein-free and the amino groups present were probably derived from a glucosamine or galactosamine polymer. Washed cell walls and intact conidia of were electrophoretically similar but cell walls of , unlike normal conidia, were phosphate-free. Mycelial ‘protoplasts’ of and and conidial ‘protoplasts’ of had pH-mobility curves characteristic of a protein surface.

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1969-07-01
2021-10-24
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