1887

Abstract

SUMMARY

Serological studies were conducted on ( 521) in an attempt to identify its agglutinating antigens.

Glycerol teichoic acid was isolated from the cell wall of 521. Isolated wall teichoic acid removed 121 and 122 antibodies from 521 antiserum, thereby demonstrating that it is an antigen involved in agglutination. 127 antibodies were not removed from antisera by the wall teichoic acid. Isolated 521 cell walls did not agglutinate in anti-112 antiserum, nor did wall teichoic acid from the same organism react with this serum.

Intracellular teichoic acid of 521 also removed agglutinating antibody from whole antisera. Gel double-diffusion experiments indicated that cell wall and intracellular teichoic acid are immunologically identical.

( 521) mucopeptide was prepared from the cell walls, and this component did not remove agglutinating antibody from antisera.

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/content/journal/micro/10.1099/00221287-57-1-105
1969-07-01
2022-01-24
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References

  1. Baddiley J. 1968; Teichoic acids and the molecular structure of bacterial walls. Proc. R. Soc. B 170331
    [Google Scholar]
  2. Baddiley J., Davison A. L. 1961; The occurrence and location of teichoic acids in lactobacilli. J. gen. Microbiol. 24:295
    [Google Scholar]
  3. Baddiley J., Buchanan J. G., Martin R. O., Rajbhandary U. L. 1962; Teichoic acid from the walls of Staphylococcus aureus h II. Location of phosphate and alanine residues. Biochem. J. 85:49
    [Google Scholar]
  4. Chung K. L., Hawirko R. Z. 1962; A serological study of cell walls of certain lactic acid bacteria. Can. J. Microbiol. 8:629
    [Google Scholar]
  5. DeMan J. C., Rogosa M., Sharpe M. E. 1960; A medium for the cultivation of lactobacilli. J. appl. Bact. 23:130
    [Google Scholar]
  6. Efthymiou C., Hansen P. A. 1962; An antigenic analysis of L. acidophilus . J. infect. Dis258
    [Google Scholar]
  7. Ellwood D. C., Tempest D. W. 1967; Teichoic acid or teichuronic acid in the walls of Bacillus subtilis var. niger grown in a chemostat. Biochem. J. 104:69
    [Google Scholar]
  8. Ellwood D. C., Tempest D. W. 1968; The teichoic acids of Bacillus subtilis var. niger and Bacillus subtilis w 23 grown in a chemostat. Biochem. J. 108:40
    [Google Scholar]
  9. Foley G. E., Wheeler S. M. 1945; Studies on the streptococci (enterococci) of Lancefield group D. I. Serologic and biochemical characteristics. Am. J. Dis. Child. 70:93
    [Google Scholar]
  10. Hansen P. A. 1965; Lactobacillus bulgaricus and its relationships. Ernahrungsforschung 10:485
    [Google Scholar]
  11. Ikawa M., Snell E. E. 1960; Cell wall composition of lactic acid bacteria. J. Biol. Chem. 235:1376
    [Google Scholar]
  12. Jones D., Shattock P. M. F. 1960; The location of the group antigen of group D streptococcus. J. gen. Microbiol. 23:335
    [Google Scholar]
  13. Juergens W. G., Sanderson A. R., Strominger J. L. 1963; Chemical basis for an immuno logical specificity of a strain of Staphylococcus aureus . J. exp. Med. 117:925
    [Google Scholar]
  14. Karakawa W. W., Krause R. M. 1966; Studies on the immunochemistry of streptococcal mucopeptide. J. exp. Med. 124:155
    [Google Scholar]
  15. Lancefield R. C. 1943; Studies on the antigenic composition of group A hemolytic streptococci. I. Effects of proteolytic enzymes on streptococcal cells. J. exp. Med. 78:465
    [Google Scholar]
  16. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. J. biol. Chem. 193:265
    [Google Scholar]
  17. Mageau R. P. 1966; A taxonomic study of Lactobacillus bulgaricus, Lactobacillus helveticus and Lactobacillus jugurti by agglutinin adsorption analysis. Master’s Thesis, University of Maryland, College Park, Md
    [Google Scholar]
  18. Miller R. H. 1957; Comparative serological and physiological studies on the subgenus Thermobacterium. Ph.D. Thesis. University of Maryland, College Park, Md
    [Google Scholar]
  19. Morse S. I. 1962; Studies on the chemistry and immunochemistry of cell walls of Staphylococcus aureus . J. exp. Med. 116:229
    [Google Scholar]
  20. Morse S. I. 1963; Isolation and properties of a group antigen of Staphylococcus albus . J. exp. Med. 117:19
    [Google Scholar]
  21. Nathenson S. G., Strominger J. L. 1962; Enzymatic synthesis and immuno-chemistry of N-acetyl-glucose-aminyl-ribitol linkages in the teichoic acids of Staphylococcus aureus strains. J. biol. Chem. 237: PC 3839
    [Google Scholar]
  22. Orla-Jensen S. 1919; The lactic acid bacteria. K. danske Vidensk. Selsk. Skr. (8 sér.) 5:81
    [Google Scholar]
  23. Orland F. J. 1950; A correlation of antigenic characteristics among certain bacteria of the lactobacillus group. J. infect. Dis. 86:68
    [Google Scholar]
  24. Ouchterlony O. 1953; Gel diffusion methods for immunological analysis. Atti VI Congr. Microbiol. Roma 2:276
    [Google Scholar]
  25. Perkins H. R. 1965; The action of hot formamide on bacterial cell walls. Biochem. J. 95:876
    [Google Scholar]
  26. Plapp P., Kandler O. 1967; Identification of l ornithine and δ amino succinyl ornithine in cell wall hydrolysates of L. cellobiosus . Nature, Lond. 213:803
    [Google Scholar]
  27. Plapp P., Schleifer K. H., Kandler O. 1967; The amino acid sequence of the mureins of lactic acid bacteria. Folia microbiol. Praha 12:205
    [Google Scholar]
  28. Sharpe M. E., Shattock P. M. F. 1952; The serological typing of group D streptococci associated with outbreaks of neonatal diarrhoea. J. gen. Microbiol. 6:150
    [Google Scholar]
  29. Sharpe M. E., Davison A. L., Baddiley J. 1964; Teichoic acids and group antigens in lactobacilli. J. gen. Microbiol. 34:333
    [Google Scholar]
  30. Strominger J. L., Izaki K., Mutsuhashi M., Tipper D. J. 1967; Peptidoglycan transpeptidase and d-alanine carboxy-peptidase : penicillin-sensitive enzymatic reactions. Fed. Proc. Fedn. Am. Socs exp. Biol. 26:9
    [Google Scholar]
  31. Tempest D. W., Dick J. W., Ellwood D. C. 1968; Influence of growth conditions on the concentration of potassium in Bacillus subtilis var. niger and its possible relationship to cellular ribonucleic acid, teichoic acid and teichuronic acid. Biochem. J. 106:237
    [Google Scholar]
  32. Torh M., Kabat E. A., Bezer A. E. 1964; Separation of teichoic acid of Staphylococcus aureus into two immunologically distinct specific polysaccharides with a and β-N-acetyl-glucosaminyl linkages respectively. Antigenicity of teichoic acids in man. J. exp. Med. 120:13
    [Google Scholar]
  33. Wade H. E., Morgan D. M. 1953; Detection of phosphate esters in paper chromatograms. Nature, Lond. 171:529
    [Google Scholar]
  34. Williams N. B. 1948; Immunologic reactions of lactobacilli. J. Am. dent. Ass. 37:403
    [Google Scholar]
  35. Williams N. B., Norris R. F., Gyorgy P. 1953; Antigenic and cultural relationships of Lactobacillus bifidus and Lactobacillus parabifidus . J. infect. Dis 92:121
    [Google Scholar]
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