1887

Abstract

SUMMARY

A mutant (Cbl-1) was isolated from 168 wild type () sporulating cultures grown on nutrient agar. This mutant differs from the parental strain as follows. (1) In nutrient broth the mutant excretes a substance which kills the cells. Therefore when grown in a mixed culture (Cbl-1 plus ), this property leads to a take-over pattern by the Cbl-1 mutant, and the spores obtained from such a culture are entirely of the mutant type. (2) The DNA of the Cbl-1 strain has a buoyant density in CsCl of 1·714g./cm. while that of the normal 168 is 1·703 g./cm. although both strains have a G + C content of 42 moles %. An explanation for the higher buoyant density of the Cbl-1 DNA has not yet been found. In addition to the base ratio data, transformation and transduction experiments indicate that Cbl-1 is a mutant of 168.

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1969-07-01
2024-10-04
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References

  1. Bott K. F., Wilson G. A. 1967; Development of competence in Bacillus subtilis transformation system. J. Bact. 94:562
    [Google Scholar]
  2. Braun W., Goodlow R. J., Kraft M., Altenberg R., Mead D. 1951; The effects of metabolites upon interactions between variants in mixed Brucella abortus populations. J. Bact. 62:75
    [Google Scholar]
  3. Douthit H. A., Halvorson H. O. 1966; Satellite deoxyribonucleic acid from Bacillus cereus T. Science, N. Y. 153:182
    [Google Scholar]
  4. Grant G., Simon M. L. 1968; Temperature-sensitive flagellar mutants in Bacillus subtilis . Bact. Proc29
    [Google Scholar]
  5. Halvorson H. O., Szulmajster J., Cohen R., Michelson A. M. 1967; Etude de l’acide désoxyribonucléique des spores de Bacillus subtilis . J. molec. Biol. 28:71
    [Google Scholar]
  6. Hoch J. A., Barat M., Anagnostopoulos C. 1967; Transformation and transduction in recombination-defective mutants of Bacillus subtilis . J. Bact. 93:1925
    [Google Scholar]
  7. Kerjan P., Marchetti J., Szulmajster J. 1967; RNA polymérase DNA dépendante des spores de B. subtilis II. Purification partielle et propriétés. Bull. Soc. Chim. biol. 49:1139
    [Google Scholar]
  8. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. molec. Biol. 3:208
    [Google Scholar]
  9. Meers J. L., Tempest D. W. 1968; The influence of extracellular products on the behaviour of mixed microbial populations in magnesium-limited chemostat cultures. J. gen. Microbiol. 52:309
    [Google Scholar]
  10. Meselson M., Stahl F. W., Vinograd J. 1957; Equilibrium sedimentation of macro-molecules in density gradient. Proc. natn. Acad. SciU.S.A 43581
    [Google Scholar]
  11. Michel J. F., Cami B., Schaeffer P. 1968; Sélection de mutants de Bacillus subtilis bloqués au début de la sporulation. II. Sélection par adaptation à une nouvelle source de carbone et par vieillissement de cultures sporulées. Annls Inst. Pasteur 114:21
    [Google Scholar]
  12. Reilly B. E. 1965; A study of the bacteriophages of Bacillus subtilis and their infectious nucleic acids. Ph.D. dissertation, Case Western Reserve University
    [Google Scholar]
  13. Schildkraut C. L., Marmur J., Doty P. 1962; Determination of base composition of deoxyribonucleic acid from its buoyant density. J. molec. Biol. 4:430
    [Google Scholar]
  14. Takahashi I. 1961; Genetic transduction in Bacillus subtilis . Biochem. biophys. Res. Commun 5:171
    [Google Scholar]
  15. Takahashi I. 1965; Transduction of sporogenesis in Bacillus subtilis . J. Bact. 89:294
    [Google Scholar]
  16. Woodward D. O. 1968; Functional and organizational properties of Neurospora mitochondrial structural protein. Fedn Proc. 27:1167
    [Google Scholar]
  17. Young F. E. 1967; Requirement of glucosylated teichoic acid for adsorption of phage in Bacillus subtilis 168. Proc. natn. Acad. SciU.S.A 582377
    [Google Scholar]
  18. Young F. E., Spizizen J. 1961; Physiologic and genetic factors affecting transformation of Bacillus subtilis . J. Bact. 81:823
    [Google Scholar]
  19. Young F. E., Brown C., Reilly B. E. 1968; Chromosomal localization of phage resistant mutants of Bacillus subtilis 168. Bact. Proc56
    [Google Scholar]
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