1887

Abstract

SUMMARY: Cell-free extracts of trophozoites of Hartmannella (Culbertson strain a-i), grown axenically, catalysed transamination between 2-oxoglutarate and a number of amino acids. Aspartic acid, alanine and serine showed the maximum activity. The L-alanine-2-oxoglutarate aminotransferase (E.C. 2.6.1.2) was purified about 15-fold by adsorption with calcium phosphate gel and elution with 0-2 M-phosphate buffer. Stability during storage, pH optima in different buffers values for alanine and 2-oxoglutarate, and the role of pyridoxal-5-phosphate are reported. -Chloromercuribenzoate, cyanide and metal-chelating agents markedly inhibited the enzyme. Among dicarboxylic acids, maleic acid had the maximum inhibitory effect. Amoe-bicidal drugs and antibiotics had no marked inhibitory effect on the enzyme.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-56-3-379
1969-06-01
2019-12-14
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-56-3-379
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error