1887

Abstract

SUMMARY

Proteins which inhibited the growth of two strains of and one strain of were isolated from the teat canal keratin of the cow. Electrophoresis of the proteins on polyacryl-amide gels suggested that they were basic. They were separated into six bands at pH 3·0 but gave only two bands at pH 4·5 and two lines of precipitin after gel diffusion and immunoelectrophoresis at pH values between 4·5 and 8·5. In the presence of the anionic polymers DNA or heparin, complexes were formed which were not inhibitory of bacterial growth. The isolated proteins and the whole teat canal keratin were completely free from lysozyme. Their role is discussed in relation to the natural defence mechanisms of the teat canal.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-56-3-365
1969-06-01
2022-01-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/56/3/mic-56-3-365.html?itemId=/content/journal/micro/10.1099/00221287-56-3-365&mimeType=html&fmt=ahah

References

  1. Adams E. W., Rickard C. G. 1963; The antistreptococcic activity of bovine teat canal keratin. Am. J. vet. Res 24:122
    [Google Scholar]
  2. Fincher M. G., Hodges H. G., Murphy J. M., Morse G. E. 1956; Mastitis research and control program. Rep. N. Y. St. vet. Coll
    [Google Scholar]
  3. Hibbitt K. G., Jones G. W. 1967; Quantitative deposition of bacteria within the teat canal of the goat. Vet. Rec 80:270
    [Google Scholar]
  4. Hibbitt K. G., Cole C. B. 1968; The antimicrobial activity of teat canal cationic proteins. Biochem. J 106:39P
    [Google Scholar]
  5. Hirsch J. G. 1958; Bactericidal action of histone. J. exp. Med 108:925
    [Google Scholar]
  6. Kern R. A., Kingkade M. J., Kern S. F., Behrens O. K. 1951; Characterisation of the action of lysozyme on Staphylococcus aureus and on Micrococcus lysodeikticus . J. Bact 61:171
    [Google Scholar]
  7. Layne E. 1957; Protein estimation by ultraviolet absorption. Meth. Enzymol 3:451
    [Google Scholar]
  8. MacMillan W. G., Hibbitt K. G. 1969; The effect of antimicrobial proteins on the fine structure of Staphylococcus aureus . J. gen. Microbiol 56:373
    [Google Scholar]
  9. Murphy J. M. 1959; The effect of certain mild stresses to the bovine teat canal on infection with Streptococcus agalactiae . Cornell Vet 49:411
    [Google Scholar]
  10. Murphy J. M., Stuart O. M. 1953; Some results of the application of Streptococcus agalactiae (Cornell 48 strain) to the bovine teat canal by means of the Hadley-Wisconsin swab technique. Cornell Vet 43:465
    [Google Scholar]
  11. Murphy J. M., Stuart O. M. 1954; The individual cow as a factor in Streptococcus agalactiae infection artificially induced by means of the Hadley-Wisconsin swab technique. Cornell Vet 44:268
    [Google Scholar]
  12. Narayan K. A., Narayan S., Kummerow F. A. 1964; A modified disc electrophoresis method for animal blood serum proteins. J. Chromatog 16:187
    [Google Scholar]
  13. Newbould F. H. S., Neave F. K. 1965; The response of the bovine mammary gland to an infusion of staphylococci. J. Dairy Res 32:163
    [Google Scholar]
  14. Padgett G. A., Hirsch J. G. 1967; Lysozyme, its absence in tears and leukocytes of cattle. Aust. J. exp. Biol. med. Sci 45:569
    [Google Scholar]
  15. Pattison I. H. 1948; Immunological studies with group B streptococci. J. Path. Bact 60:219
    [Google Scholar]
  16. Plastridge W. N. 1958; Bovine mastitis: A review. J. Dairy Sci 41:1141
    [Google Scholar]
  17. Scarnes R. C., Watson D. W. 1957; Antimicrobial factors of normal tissues and fluids. Bact. Rev 21:273
    [Google Scholar]
  18. Scheidegger J. J. 1955; Une microméthode de l’immunoelectrophorèse. Intern. Arch. Allergy appl. Immunol 7:103
    [Google Scholar]
  19. Shugar D. 1952; The measurement of lysozyme activity and the ultraviolet inactivation of lysozyme. Biochim. biophys. Acta 8:302
    [Google Scholar]
  20. Treese J. M., Morse G. E., Levy C. 1966; Lipid analysis of bovine teat canal keratin. J. Dairy Sci 49:1240
    [Google Scholar]
  21. Zeya H. I., Spitznagel J. K. 1966; Cationic proteins of polymorphonuclear leukocyte lysosomes. J. Bact 91:750
    [Google Scholar]
  22. Zubay G., Wilkins M. H. F. 1962; An X-ray diffraction study of histone and protamine in isolation and in combination with DNA. J. molec. Biol 4:444
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-56-3-365
Loading
/content/journal/micro/10.1099/00221287-56-3-365
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error