SUMMARY: Proteins which inhibited the growth of two strains of and one strain of were isolated from the teat canal keratin of the cow. Electrophoresis of the proteins on polyacryl-amide gels suggested that they were basic. They were separated into six bands at pH 3.0 but gave only two bands at pH 4.5 and two lines of precipitin after gel diffusion and immunoelectrophoresis at pH values between 4.5 and 8.5. In the presence of the anionic polymers DNA or heparin, complexes were formed which were not inhibitory of bacterial growth. The isolated proteins and the whole teat canal keratin were completely free from lysozyme. Their role is discussed in relation to the natural defence mechanisms of the teat canal.


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