1887

Abstract

SUMMARY

Phenol-purified RNA was prepared from and characterized by sucrose-gradient centrifugation. The patterns obtained indicate that possesses a ribosomal RNA complement similar to that of This is contrary to previous claims that the 23S component is missing. This species of ribosomal RNA was demonstrated to be more labile than the corresponding component in and variations in yields produced by changes in extraction procedures are described.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-56-3-345
1969-06-01
2024-12-03
Loading full text...

Full text loading...

/deliver/fulltext/micro/56/3/mic-56-3-345.html?itemId=/content/journal/micro/10.1099/00221287-56-3-345&mimeType=html&fmt=ahah

References

  1. Britten R. J., Roberts R. B. 1960; High resolution gradient sedimentation analysis. Science, N. Y 131:32
    [Google Scholar]
  2. Egger E., Rapoport S. 1963; Role of non-covalent bonds for the holding, activation and release of mitochondrial enzymes. Nature, Lond 200:240
    [Google Scholar]
  3. Friedman D. I., Pollara B., Gray E. D. 1966; Structural studies of the ribosomes of Rhodopseudomonas spheroides . J. molec. Biol 22:53
    [Google Scholar]
  4. Green M. H. 1966; Inactivation of the prophage Lambda repressor without induction. J. molec. Biol 16:134
    [Google Scholar]
  5. Lascelles J. 1959; The synthesis of enzymes concerned in bacteriochlorophyll formation in growing cultures of Rhodopseudomonas spheroides . J. gen. Microbiol 23:487
    [Google Scholar]
  6. Lessie T. G. 1965; The atypical ribosomal RNA complement of Rhodopseudomonas spheroides . J. gen. Microbiol 39:311
    [Google Scholar]
  7. Midgley J. E. M. 1965a; Effects of different extraction procedures on the molecular characteristics of bacterial ribosomal ribonucleic acid. Biochim. biophys. Acta 95:232
    [Google Scholar]
  8. Midgley J. E. M. 1965b; Studies on the structure of 23S ribosomal ribonucleic acid from Escherichia coli . Biochim. biophys. Acta 108:348
    [Google Scholar]
  9. Midgley J. E. M. 1965c; The estimation of polynucleotide chain length by a chemical method. Biochim. biophys. Acta 108:340
    [Google Scholar]
  10. Osawa S. 1968; Ribosome formation and structure. A. Rev. Biochem 37:109
    [Google Scholar]
  11. Takanami M. 1967; Analysis of the 5′-terminal nucleotide sequences of ribonucleic acids. I. The 5′-termini of Escherichia coli ribosomal RNA. J. molec. Biol 23:135
    [Google Scholar]
  12. Yamashita J., Kamen M. D. 1968; Observations on the nature of pulse-labelled RNA from photosynthetically or heterotrophically grown Rhodospirillum rubrum . Biochim. biophys. Acta 161:162
    [Google Scholar]
/content/journal/micro/10.1099/00221287-56-3-345
Loading
/content/journal/micro/10.1099/00221287-56-3-345
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error