Properties of and Providence Spheroplasts Free

Abstract

SUMMARY

strain 13 and Providence strain 9211 were converted to osmotically sensitive spheroplasts by growth in the presence of penicillin or glycine in sucrose-supplemented or unsupplemented medium. Lysozyme in the presence of tris and ethylenediaminetetra-acetic acid (EDTA) converted both these strains to spheroplasts. Penicillin spheroplasts could be stabilized with sucrose, streptomycin, spermine or di-valent cations. Electron micrographs of these spheroplasts demonstrated that the outer layer of the bacterial cell wall was retained. Penicillin-induced spheroplasts possessed receptor sites for bacteriophages.

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1969-05-01
2024-03-29
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