@article{mbs:/content/journal/micro/10.1099/00221287-56-2-257, author = "Van Rensburg, A. J.", title = "Properties of Proteus mirabilis and Providence Spheroplasts", journal= "Microbiology", year = "1969", volume = "56", number = "2", pages = "257-264", doi = "https://doi.org/10.1099/00221287-56-2-257", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-56-2-257", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "SUMMARY Proteus mirabilis strain 13 and Providence strain nctc9211 were converted to osmotically sensitive spheroplasts by growth in the presence of penicillin or glycine in sucrose-supplemented or unsupplemented medium. Lysozyme in the presence of tris and ethylenediaminetetra-acetic acid (EDTA) converted both these strains to spheroplasts. Penicillin spheroplasts could be stabilized with sucrose, streptomycin, spermine or di-valent cations. Electron micrographs of these spheroplasts demonstrated that the outer layer of the bacterial cell wall was retained. Penicillin-induced spheroplasts possessed receptor sites for bacteriophages.", }