1887

Abstract

SUMMARY: strain 13 and Providence strain 9211 were converted to osmotically sensitive spheroplasts by growth in the presence of penicillin or glycine in sucrose-supplemented or unsupplemented medium. Lysozyme in the presence of tris and ethylenediaminetetra-acetic acid (EDTA) converted both these strains to spheroplasts. Penicillin spheroplasts could be stabilized with sucrose, streptomycin, spermine or di-valent cations. Electron micrographs of these spheroplasts demonstrated that the outer layer of the bacterial cell wall was retained. Penicillin-induced spheroplasts possessed receptor sites for bacteriophages.

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/content/journal/micro/10.1099/00221287-56-2-257
1969-05-01
2020-01-19
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