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The properties of a temperature-sensitive mutant of Bacillus subtilis in which DNA synthesis is specifically and reversibly inhibited above 40° are described. When incubated at 45° there was no detectable breakdown of DNA in the mutant and the synthesis of deoxynucleoside triphosphates was normal. The temperature-sensitive element affects DNA synthesis in vivo but not in vitro and (unlike an initiator) it appears to be needed for DNA synthesis throughout the replication cycle.
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