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SUMMARY: When the growth of surface cultures of Mycobacterium phlei was limited by nitrogen or sulphur, the organisms synthesized both glycogen and lipid as endogenous reserves. Equal weights of glycogen and lipid accumulated intracellularly which, combined, may account for 50 % of the cell dry weight. Both storage materials also accumulated when growth was inhibited by chloramphenicol or p-fluorophenylalanine in otherwise nutritionally adequate media. In the absence of exogenous carbon substrate, the glycogen and lipid reserves were utilized as energy and carbon for nitrogen incorporation and continued growth. Evidence is presented which suggests that glycogen may be the preferred endogenous reserve in M. phlei.
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