@article{mbs:/content/journal/micro/10.1099/00221287-55-2-201, author = "Kingsbury, D. T. and Fanning, G. R. and Johnson, K. E. and Brenner, D. J.", title = "Thermal Stability of Interspecies Neisseria DNA Duplexes", journal= "Microbiology", year = "1969", volume = "55", number = "2", pages = "201-208", doi = "https://doi.org/10.1099/00221287-55-2-201", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-55-2-201", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "SUMMARY: The thermal stability of interspecies DNA duplexes is markedly increased by raising the incubation temperature. When the DNA reassociation reactions are carried out at 75° in 0·12 m-phosphate buffer the thermal denaturation temperature of the reassociated product is almost identical to that of the native DNA, indicating that only DNA segments of very similar nucleotide sequence are associating. The genus Neisseria very clearly forms three groups based on the relatedness of their DNA to that of N. meningitidis; the ‘pathogenic’ Neisseria which have at least 80 % of their nucleotide sequences similar; the ‘non-pathogenic’ Neisseria which share only 8 to 15 %; and N. catarrhalis which shows no relatedness.", }