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1887

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Volume 55, Issue 2

Transformation of Nutritionally Deficient Mutants of Free

Abstract

SUMMARY: Reversions to wild type occurred when conidia of a number of biochemically deficient mutants of were treated with DNA from the wild-type strain. No reversions or wild-type colonies were obtained when an equivalent number of conidia from deficient strains were either treated with the same DNA as the recipient, or were plated without any DNA treatment. Increase in the percentage of transformation was observed up to 6 g./ml. of donor DNA. The transforming activity of the donor DNA was found to be inhibited by the action of u.v. radiation, heat and DNase. The frequency of transformation was low which could be attributed to the method of extraction of DNA which involved crushing of cells in the presence of alumina, possibly breaking the DNA into small fragments thus making it biologically less active.

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© Society for General Microbiology, 1969
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/content/journal/micro/10.1099/00221287-55-2-195
1969-02-01
2025-05-24
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References

  1. Bucknall R. A., Morton A. G. 1964; Attempted genetic transformation in Penicillium chryso-genum . Nature; Lond.: 20 157
     [Google Scholar]
  2. Clutterbuck P. W., Lovell R., Raistrick H. 1932; XXVI. The formation from glucose by members of the Penicillium chrysogenum series of a pigment, an alkali-soluble protein and penicillin-the antibacterial substance of Fleming. Biochem. J 26:1907
     [Google Scholar]
  3. Gwinn D. D., Thorne C. B. 1964; Transformation of Bacillus licheniformis . J. Bact 87:519
     [Google Scholar]
  4. Hotchkiss R. D. 1951; Transfer of penicillin resistance in pneumococci by deoxyribonucleate derived from resistant cultures. Cold Spring Harb. Symp. quant. Biol 16:457
     [Google Scholar]
  5. Leonard C. G., Corley D., Cole R. M. 1966; Transformation of auxotrophic mutants of group H streptococci. Science, N.Y. 152:1255
     [Google Scholar]
  6. Lerman L. S., Tolmach L. J. 1959; Genetic transformation. II. The significance of damage to the DNA molecule. Biochim. biophys, Acta 33:371
     [Google Scholar]
  7. Marmur J., Fluke D. J. 1955; Uniformity of ionizing radiation on several transforming factors of pneumococcus. Archs. Biochem. Biophys 57:506
     [Google Scholar]
  8. Mishra A. K., Nandi P. 1959; Ultraviolet induced biochemical mutations in Aspergillus niger. . Sci. Cult 25:81
     [Google Scholar]
  9. Shamoian C. A., Canzanelli A., Melrose J. 1961; Back mutation of a Neurospora crassa mutant by a nucleic acid complex from wild strain. Biochim. biophys, Acta 47:208
     [Google Scholar]
  10. Shockley T. E., Tatum E. L. 1962; A search for genetic transformation in Neurospora crassa. . Biochim. biophys, Acta 61:567
     [Google Scholar]
  11. Spizizen J. 1958; Transformation of biochemically deficient strain of Bacillus subtilis by deoxyribonucleate. Proc. natn. Acad. Sci. U.S.A 44:1072
     [Google Scholar]
/content/journal/micro/10.1099/00221287-55-2-195
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Transformation of Nutritionally Deficient Mutants of Aspergillus Niger
Microbiology 55, 195 (1969); https://doi.org/10.1099/00221287-55-2-195
/content/journal/micro/10.1099/00221287-55-2-195
/content/journal/micro/10.1099/00221287-55-2-195
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