1887

Abstract

SUMMARY Explants of young tumour tissue from several Brassica species infected with plasmodia gave rapid callus growth on medium containing 2,4-dichlorophenoxyacetic acid and coconut milk. Degeneration of the callus followed as resting spores were formed. The resting spores germinated and primary plasmodia and zoosporangia developed in some cells, then secondary plasmodia reappeared and normal callus growth was resumed; thereafter all stages of the parasite life-cycle were present in active cells. This situation has been maintaned for over 18 months by 8weekly transfer of calluses to new medium. Callus clones containing only vegetative plasmodia were established on a coconut-milk + 2, 4-dichloro-phenoxyacetic acid medium. Sporogenesis did not occur readily in these clones unless callus was transferred to a kinetin + -naphthylacetic acid medium or to other media which did not contain 2,4-dichlorophenoxyacetic acid. Stages of the . life-cycle found in callus appeared similar to those in intact hosts, while zoosporangia were formed in the root hairs of organ cultures infected with callus-produced resting spores. It was concluded that on suitable media may be maintained in complete balance with host callus.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-55-1-9
1969-01-01
2021-10-16
Loading full text...

Full text loading...

/deliver/fulltext/micro/55/1/mic-55-1-9.html?itemId=/content/journal/micro/10.1099/00221287-55-1-9&mimeType=html&fmt=ahah

References

  1. Channon A. G., Flint A. E., Hinton R. A. L. 1964; A quantitative laboratory method for inoculating cabbage seedlings with Plasmodiophora brassicae Woron. Ann. appl. Biol 54:71
    [Google Scholar]
  2. Constabel F. 1957; Ernährungsphysiologische und manometrische Untersuchungen zur Gewebekultur der Gymnosporangium-Gallen von Juniperus-Arten. Biol. Zbl 76:385
    [Google Scholar]
  3. Cutter V. M. 1959; Studies on the isolation and growth of plant rusts in host tissue cultures and upon synthetic media. I. Gymnosporangium. Mycologia 51:248
    [Google Scholar]
  4. Cutter V. M. 1960; Studies on the isolation and growth of plant rusts in host tissue cultures and upon synthetic media. II. Uromyces ari-triphylli . Mycologia 52:726
    [Google Scholar]
  5. Hildebrandt A. C. 1962; Tissue and single cell cultures of higher plants as a basic experimental method. In Modern Methods of Plant Analysis Linskens H. F., Tracey M. V. Ed. by 5 pp. 383 Berlin: Springer;
    [Google Scholar]
  6. Hotson H. H., Cutter V. M. 1951; The isolation and culture of Gymnosporangium juniperivirginhmae Schw. Proc. natn. Acad. Sci. U.S.A 37:400
    [Google Scholar]
  7. Ingram D. S. 1967; The expression of R-gene resistance to Phytophthora infestans in tissue cultures of Solanum tuberosum . J. gen. Microbiol 49:99
    [Google Scholar]
  8. Karling J. S. 1942 The Plasmodiophorales Karling, J. S. Columbia University; New York:
    [Google Scholar]
  9. Köle A. P., Philipsen P. J. J. 1956; Over de vatbarrheid van niet-kruisbloemige planten voor het zoosporangium-stadium van Plasmodiophora brassicae Woron. Tijdschr. PlZiekt 62:167
    [Google Scholar]
  10. Maheshwari R., Hildebrandt A. C., Allen P. J. 1968; Factors affecting the growth of rust fungi on host tissue cultures. Bot. Gaz 128:153
    [Google Scholar]
  11. Morel G. 1944; Le développement du mildiou sur des tissus de Vigne cultures in vitro . C. r. hebd. Séanc. Acad. Sci., Paris 218:50
    [Google Scholar]
  12. Mosse B. 1962; The establishment of vesicular-arbuscular mycorrhiza under aseptic conditions. J. gen. Microbiol 27:509
    [Google Scholar]
  13. Rossetti V., Morel G. 1958; Le développement du Puccinia antirrhini sur tissu de muflier cultivés in vitro . C. r. hebd. Séanc. Acad. Sci., Paris 247:1893
    [Google Scholar]
  14. Smith J. E. 1956; Host parasite physiology in relation to clubroot disease of Crucifers. Diss. Abstr 16:440
    [Google Scholar]
  15. Strandberg J. O., Williams P. H., Yukawa Y. 1966 Phytopathology 56:903 abstract
    [Google Scholar]
  16. Street H. E., Henshaw G. G. 1966; Introduction and methods employed in plant tissue culture. In Cells and Tissues in Culture Willmer E. N. Ed. by 3 p. 459 London: Academic Press;
    [Google Scholar]
  17. Tulecke W., Weinstein L. H., Rutner A., Laurencot H. J. 1961; The biochemical composition of coconut water as related to its use in plant tissue culture. Contr. Boyce Thompson Inst 21:115
    [Google Scholar]
  18. Williams P. H., Yukawa Y. B. 1967; Ultrastructural studies on the host parasite relations of Plasmodiophora brassicae . Phytopathology 57:682
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-55-1-9
Loading
/content/journal/micro/10.1099/00221287-55-1-9
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error