1887

Abstract

Summary: Using non-flagellate ( ) mutants of gn-complex antigenic derivatives of a stable phase-1 strain , the positions of the antigenic specificity-determining sections on the genetic map of , a structural gene for flagellar protein, were located by establishing a system for selecting intra- recombinants. Before the recombination experiments, a factor analysis of the gn-complex antigens of the strains was made by cross-absorption tests: nine factors were detected.

P22 phage-mediated transductions were carried out between different pairs of -linked mutants, whose -alleles were different and whose shes were on opposite sides of The transductants, present as swarms in semi-solid medium, were isolated and the composition of their flagellar antigens examined with specific antisera. Among 1366 transductants obtained, 26 clones were shown to have ‘recombinant antigens’ carrying some factors of one or both of the parental-type antigens. With several ‘recombinant antigens’, some new specificities with weak antigenicity were detected. The flagellar protein, ‘flagellin’, of an ‘antigen recombinant’ was proved to be a recombinant of the two parental flagellins by finger-printing analysis of tryptic digests.

On the assumption that these ‘antigen recombinants’ resulted from a single cross-over within relative positions of the antigenic specificitydetermining sections within donor and recipient with regard to the crossover point were obtained for individual ‘antigen recombinants’. Summarizing the relative positions thus inferred, it was shown that each of the antigenic specificity-determining sections maps as a unit; and that as a whole they form a linear array within . Sections specifying some tryptic peptides of flagellins were also mapped within .

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1969-01-01
2021-10-21
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