1887

Abstract

SUMMARY: Antisera to nine strains of were prepared in rabbits, chickens and turkeys, the response of individual rabbits or birds varying widely. Minor antigenic differences between some of the strains were shown in metabolic inhibition (m.i.) tests with rabbit antisera. The strains could be divided into at least three subtypes. In general, antibody diminished so that it was barely detectable 6 months after inoculation.

Antibody titres measured by m.i. were lower in chicken and turkey antisera than in rabbit antisera. The choice of the mycoplasma strain to be used in the m.i. test for the measurement of antibody in the bird sera was of fundamental importance since not all strains were capable of detecting antibody; strain T37 was the most useful.

Antibody measured by m.i. was not destroyed by repeated freezing and thawing of antisera, and the antibody titres were not, in general, increased by the addition of unheated guinea-pig serum to the tests. The titres of antibody which inhibited metabolism of the mycoplasma strains were closely similar to those which inhibited their growth in liquid medium. On solid medium, however, some rabbit antisera with high m.i. antibody titres did not inhibit or inhibited poorly the development of colonies of the homologous strains. Only one of the nine rabbit antisera inhibited the development of colonies of all the strains, a feature of importance in mycoplasma identification.

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/content/journal/micro/10.1099/00221287-55-1-127
1969-01-01
2021-07-25
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References

  1. Adler H. E. 1953; Preliminary report on the slide agglutination test for pleuropneumonia-like agents associated with infectious sinusitis of turkeys and chronic respiratory disease of chickens. SWest. Vet 6:362
    [Google Scholar]
  2. Barker L. F., Patt J. K. 1967; Role of complement in immune inactivation of Mycoplasma gallisepticum . J. Bact 94:403
    [Google Scholar]
  3. Clyde W. A. Jun 1964; Mycoplasma species identification based upon growth inhibition by specific antisera. J. Immunol 92:958
    [Google Scholar]
  4. Dierks R. E., Newman J. A., Pomeroy B. S. 1967; Characterization of avian Mycoplasma. Ann. N. Y. Acad. Sci 143:170
    [Google Scholar]
  5. Dinter Z., Danielsson D., Bakos K. 1965; Differentiation of porcine mycoplasma strains. J. gen. Microbiol 41:77
    [Google Scholar]
  6. Edward D. G. ff., Kanarek A. D. 1960; Organisms of the pleuropneumonia group of avian origin: their classification into species. Ann. N. Y. Acad. Sci 79:696
    [Google Scholar]
  7. Fabricant J. 1960; Serological studies of avian pleuropneumonia-like organisms (PPLO) with Edward’s technique. Avian Dis 4:505
    [Google Scholar]
  8. Fallon R. J., Jackson D. K. 1967; The relationship between a rodent mycoplasma, Mycoplasma pulmonis, and certain mycoplasmas isolated from tissue cultures inoculated with material from patients with leukaemia. Lab. Anim 1:55
    [Google Scholar]
  9. Hayflick L. 1965; Tissue cultures and mycoplasmas. Texas Rep. Biol. Med 23: suppl 1 285
    [Google Scholar]
  10. Hayflick L., Stanbridge E. 1967; Isolation and identification of Mycoplasma from human clinical materials. Ann. N. Y. Acad. Sci 143:608
    [Google Scholar]
  11. Jungherr E. L., Luginbuhl R. E., Jacobs R. E. 1953; Pathology and serology of air sac infection. Proc. Am. vet. med. Ass p. 303
    [Google Scholar]
  12. Manchee R. J., Taylor-Robinson D. 1968; Haemadsorption and haemagglutination by mycoplasmas. J. gen. Microbiol 50:465
    [Google Scholar]
  13. Newnham A. G. 1964; The haemagglutination-inhibition (HI) test and a study of its use in experimental avian respiratory mycoplasmosis. Res. vet. Sci 5:245
    [Google Scholar]
  14. Purcell R. H., Wong D., Chanock R. M., Taylor-Robinson D., Canchola J., Valdesuso J. 1967; Significance of antibody to Mycoplasma as measured by metabolic-inhibition techniques. Ann. N. Y. Acad. Sci 143:664
    [Google Scholar]
  15. Smith S. C., Dunlop W. R., Strout R. G. 1966; Effect of culture medium on antigenic structure of Mycoplasma. Avian Dis io:173
    [Google Scholar]
  16. Taylor-Robinson D., Purcell R. H. 1966; Mycoplasmas of the human urogenital tract and oropharynx and their possible role in disease: a review with some recent observations. Proc. roy. Soc. Med 59:1112
    [Google Scholar]
  17. Taylor-Robinson D., Canchola J., Fox H., Chanock R. M. 1964; A newly identified oral mycoplasma (M. orale) and its relationship to other human mycoplasmas. Am. J. Hyg 80:135
    [Google Scholar]
  18. Taylor-Robinson D., Purcell R. H., Wong D. C., Chanock R. M. 1966; A colour test for the measurement of antibody to certain mycoplasma species based upon the inhibition of acid production. J. Hyg., Cambs 64:91
    [Google Scholar]
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