SUMMARY: Pairs of non-tetracycline-producing mutants of or were grown side by side on agar. Their ability to produce antibiotic by cosynthesis was tested by placing a strip of agar cut from the combined culture on plates containing . The activity was revealed as an inhibition halo formed on , opposite one or other mutant strain. The strain surrounded by the halo was considered as a converter of an intermediate product secreted by the other strain. Two types of mutants were observed: a rare type probably affecting the main pathway of antibiotic biosynthesis, and a more frequent type probably affecting some regulatory process.


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  1. Alikhanian S. I., Orlova N. V., Mindlin S. Z., Zeitzeva Z. M. 1961; Genetic control of oxytetracycline biosynthesis. Nature; Lond: 189939
    [Google Scholar]
  2. Bu’lock J. D. 1965 The Biosynthesis of Natural Products: an Introduction to Secondary Metabolism London: McGraw-Hill Publ. Co. Ltd;
    [Google Scholar]
  3. Lancini G. C., Sensi P. 1964; Isolation of 2-acetyl-2-decarboxamido tetracycline from culture of Streptomyces psammoticus . Experientia 20:83
    [Google Scholar]
  4. McCormick J. R. D., Hirsch U., Sjolander N. O., Doerschuk A. P. 1960; Cosynthesis of tetracycline by pairs of Streptomyces aureofaciens mutants. J. Am. chem. Soc 82:5006
    [Google Scholar]
  5. McCormick J. R. D., Sjolander N. O., Hirsch U. 1961; Production of tetracyclines. U.S. Patent. 2:998–352
    [Google Scholar]

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